|Title||Subdiffraction-Limit Two-Photon Fluorescence Microscopy for GFP-Tagged Cell Imaging|
|Publication Type||Journal Article|
|Year of Publication||2009|
|Authors||Li, QF, Wu, SSH, Chou, KC|
|Type of Article||Article|
|Keywords||CAVEOLAE, NANOSCOPY, PROTEIN, RESOLUTION, STATES, STED MICROSCOPY, STIMULATED-EMISSION-DEPLETION|
We report applications of two-photon excitation fluorescence (2PEF) microscopy with subdiffraction-limit resolution for green-fluorescent-protein-tagged cell imaging. The microscope integrates 2PEF microscopy and stimulated emission depletion microscopy in one microscope that has the benefits of both techniques: intrinsic three-dimensional resolution, confined photobleaching, and subdiffraction-limit resolution. The subdiffraction-limit resolution was demonstrated by resolving green-fluorescent-protein-tagged caveolar vesicles located within a distance shorter than the diffraction limit of a regular 2PEF microscope, which is similar to 250 nm even with the best optics. The full width at half-maximum of the effective point-spread function for the 2PEF microscope was estimated to be similar to 54 nm.
|URL||<Go to ISI>://000272765400020|