@article {2500,
	title = {Subdiffraction-Limit Two-Photon Fluorescence Microscopy for GFP-Tagged Cell Imaging},
	journal = {Biophysical Journal},
	volume = {97},
	number = {12},
	year = {2009},
	note = {ISI Document Delivery No.: 532QVTimes Cited: 1Cited Reference Count: 33Li, Qifeng Wu, Sherry S. H. Chou, Keng C.},
	month = {Dec},
	pages = {3224-3228},
	type = {Article},
	abstract = {We report applications of two-photon excitation fluorescence (2PEF) microscopy with subdiffraction-limit resolution for green-fluorescent-protein-tagged cell imaging. The microscope integrates 2PEF microscopy and stimulated emission depletion microscopy in one microscope that has the benefits of both techniques: intrinsic three-dimensional resolution, confined photobleaching, and subdiffraction-limit resolution. The subdiffraction-limit resolution was demonstrated by resolving green-fluorescent-protein-tagged caveolar vesicles located within a distance shorter than the diffraction limit of a regular 2PEF microscope, which is similar to 250 nm even with the best optics. The full width at half-maximum of the effective point-spread function for the 2PEF microscope was estimated to be similar to 54 nm.},
	keywords = {CAVEOLAE, NANOSCOPY, PROTEIN, RESOLUTION, STATES, STED MICROSCOPY, STIMULATED-EMISSION-DEPLETION},
	isbn = {0006-3495},
	url = {<Go to ISI>://000272765400020},
	author = {Li, Q. F. and Wu, S. S. H. and Chou, K. C.}
}
