|Title||Understanding viral neuraminidase inhibition by substituted difluorosialic acids|
|Publication Type||Journal Article|
|Year of Publication||2015|
|Authors||Weck, S, Robinson, K, Smith, MR, Withers, SG|
Mechanism-based inhibition of influenza neuraminidases by difluorosialic acids (DFSA) is not only rendered highly specific by incorporation of 4-amino or 4-guanidine substituents but also the half-life for reactivation is greatly increased. Measurement of rate constants for spontaneous hydrolysis of a series of such substituted DFSAs reveals, surprisingly, that inherent inductive effects play very little role in this rate reduction and that interactions with the enzyme are more important.