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Improvement of the Expression Level of beta-Glucosidase from Agrobacterium sp in Escherichia coli by Rare Codon Optimization

TitleImprovement of the Expression Level of beta-Glucosidase from Agrobacterium sp in Escherichia coli by Rare Codon Optimization
Publication TypeJournal Article
Year of Publication2013
AuthorsShim, J-H, Withers, SG
JournalFOOD SCIENCE AND BIOTECHNOLOGY
Volume22
Pagination269-273
Date PublishedFEB
ISSN1226-7708
Abstract

To achieve high level expression of beta-glucosidase from Agrobacterium sp. (Abg) in an Escherichia coli expression system, 6 rare codons at +3, +11, +158, +308, +314, and +318 of abg gene were replaced with favored codons using site-directed mutagenesis. The rare codon replacements of +3 (CCC) and +11 (CCC) positions enhanced the expression level of Abg by 2- and 3.6-fold, respectively. The double mutant, Abg-DM, where both of +3 and +11 rare codon positions were modified showed a 5.2-fold higher expression level than the original abg gene. Circular dichroism (CD) spectra of the over-expressed Abg and original Abg enzymes were identical, indicating that Abg was properly folded during over-expression.

DOI10.1007/s10068-013-0036-6