@article { ISI:000315417400036,
	title = {Improvement of the Expression Level of beta-Glucosidase from Agrobacterium sp in Escherichia coli by Rare Codon Optimization},
	journal = {FOOD SCIENCE AND BIOTECHNOLOGY},
	volume = {22},
	number = {1},
	year = {2013},
	month = {FEB},
	pages = {269-273},
	abstract = {To achieve high level expression of beta-glucosidase from Agrobacterium sp. (Abg) in an Escherichia coli expression system, 6 rare codons at +3, +11, +158, +308, +314, and +318 of abg gene were replaced with favored codons using site-directed mutagenesis. The rare codon replacements of +3 (CCC) and +11 (CCC) positions enhanced the expression level of Abg by 2- and 3.6-fold, respectively. The double mutant, Abg-DM, where both of +3 and +11 rare codon positions were modified showed a 5.2-fold higher expression level than the original abg gene. Circular dichroism (CD) spectra of the over-expressed Abg and original Abg enzymes were identical, indicating that Abg was properly folded during over-expression.},
	issn = {1226-7708},
	doi = {10.1007/s10068-013-0036-6},
	author = {Shim, Jae-Hoon and Withers, Stephen G.}
}
