Research & Teaching Faculty

X-ray magnetic circular dichroism of Pseudomonas aeruginosa nickel(II) azurin.

TitleX-ray magnetic circular dichroism of Pseudomonas aeruginosa nickel(II) azurin.
Publication TypeJournal Article
Year of Publication2004
AuthorsFunk, T, Kennepohl, P, Di Bilio, AJ, Wehbi, Wa, Young, AT, Friedrich, S, Arenholz, E, Gray, HB, Cramer, SP
JournalJ. Am. Chem. Soc.
KeywordsAzurin, Azurin: chemistry, circular dichroism, Circular Dichroism: methods, copper, Copper: chemistry, CRYSTALLOGRAPHY, LIGANDS, Magnetics, models, MOLECULAR, Molecular Conformation, NICKEL, Nickel: chemistry, PDF, Pseudomonas aeruginosa, Pseudomonas aeruginosa: chemistry, X-RAY, X-Rays

We show that X-ray magnetic circular dichroism (XMCD) can be employed to probe the oxidation states and other electronic structural features of nickel active sites in proteins. As a calibration standard, we have measured XMCD and X-ray absorption (XAS) spectra for the nickel(II) derivative of Pseudomonas aeruginosa azurin (NiAz). Our analysis of these spectra confirms that the electronic ground state of NiAz is high-spin (S = 1); we also find that the L(3)-centroid energy is 853.1(1) eV, the branching ratio is 0.722(4), and the magnetic moment is 1.9(4) mu(B). Density functional theory (DFT) calculations on model NiAz structures establish that orbitals 3d(x2-y2) and 3d(z2) are the two valence holes in the high-spin Ni(II) ground state, and in accord with the experimentally determined orbital magnetic moment, the DFT results also demonstrate that both holes are highly delocalized, with 3d(x2-y2) having much greater ligand character.