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X-ray crystal structures of rabbit N-acetylglucosaminyltransferase I (GnT I) in complex with donor substrate analogues

TitleX-ray crystal structures of rabbit N-acetylglucosaminyltransferase I (GnT I) in complex with donor substrate analogues
Publication TypeJournal Article
Year of Publication2006
AuthorsGordon, RD, Sivarajah, P, Satkunarajah, M, Ma, D, Tarling, CA, Vizitiu, D, Withers, SG, Rini, JM
JournalJOURNAL OF MOLECULAR BIOLOGY
Volume360
Pagination67-79
Date PublishedJUN 30
ISSN0022-2836
Abstract

The Golgi-resident glycosyltransferase, UDP-N-acetyl-D-glucosamine:alpha-3-D-mannoside beta-1,2-N-acetylglucosaminyltransferase I (GnT I), initiates the conversion of high-mannose oligosaccharides to complex and hybrid structures in the biosynthesis of N-linked glycans. Reported here are the X-ray crystal structures of GnT I in complex with UDP-CH2-GIcNAc (a non-hydrolyzable C-glycosidic phosphonate), UDP-2-deoxy-2-fluoro-glucose, UDP-glucose and UDP. Collectively, these structures provide evidence for the importance of the GlcNAc moiety and its N-acetyl group in donor substrate binding, as well as insight into the role played by the flexible 318-330 loop in substrate binding and product release. In addition, the UDP-CH2-GlcNAc complex reveals a well-defined glycerol molecule poised for nucleophilic attack on the C1 atom of the donor substrate analogue. The position and orientation of this glycerol molecule have allowed us to model the binding of the Man alpha 1, 3Man beta 1 moiety of the acceptor substrate and, based on the model, to suggest a rationalization for the main determinants of GnT I acceptor specificity. (c) 2006 Elsevier Ltd. All rights reserved.

DOI10.1016/j.jmb.2006.04.058