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Ultraviolet resonance Raman spectroscopy of locked single-stranded oligo(dA) reveals conformational implications of the locked ribose in LNA

TitleUltraviolet resonance Raman spectroscopy of locked single-stranded oligo(dA) reveals conformational implications of the locked ribose in LNA
Publication TypeJournal Article
Year of Publication2009
AuthorsKonorov, SO, Schulze, HG, Addison, CJ, Haynes, CA, Blades, MW, Turner, RFB
JournalJournal of Raman Spectroscopy
Volume40
Pagination1162-1171
Date PublishedSep
Type of ArticleArticle
ISBN Number0377-0486
Abstract

We report here the first UV resonance Raman spectroscopic (UVRRS) study on locked nucleic acid (LNA) oligomers. Locking a base in nucleic acid (NA) oligomers produces a conformational change in the glycosyl bond between backbone and base. We present evidence of this change in LNAs when compared to their natural analogs using UVRRS. Wavenumber downshifts and peak amplitude increases, especially of the similar to 1481 cm(-1) peak that is a spectral marker for part of the glycosyl bond, correlate with the fraction of locked bases when single-stranded oligomers incorporating up to three locked bases were examined. By varying the position of the locked base within a fixed length sequence, we conclude that one, or at most two bases, on either side of the lock is affected. We further conclude from these data, and previously published reports,that the conformation of LNA is determined by imidazole-imidazole and pyrimidine-pyrimidine repulsion and imidazole-pyrimidine attraction in contrast to dispersion attraction-dependent aggregation in the B conformation of DNA. Copyright (C) 2009 John Wiley & Sons, Ltd.

DOI10.1002/jrs.2251