Title | On the structures of filamentous bacteriophage Ff (fd, f1, M13) |
Publication Type | Journal Article |
Year of Publication | 2008 |
Authors | Straus, SK, Scott, WRP, Symmons, MF, Marvin, DA |
Journal | European Biophysics Journal with Biophysics Letters |
Volume | 37 |
Pagination | 521-527 |
Date Published | Apr |
ISBN Number | 0175-7571 |
Abstract | The filamentous bacteriophage (Inovirus) strain Ff (fd, f1, M13) is widely used in molecular biophysics as a simple model system. A low resolution molecular model of the fd protein coat has been reported, derived from iterative helical real space reconstruction of cryo-electron micrographs (cryoEM). This model is significantly different from the model previously derived from X-ray fibre diffraction and solid-state NMR. We show that the cryoEM model agrees neither with solid-state NMR data nor with X-ray fibre diffraction data of fd, and has some puzzling structural features, for instance nanometre holes through the protein coat. We refine the cryoEM model against the X-ray data, and find that the model after refinement closely approximates the model derived directly from X-ray fibre diffraction and solid-state NMR data. We suggest possible reasons for the differences between the models derived from cryoEM and X-ray diffraction. |
URL | <Go to ISI>://000254235700018 |
