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Strategies for Modulating the pH-Dependent Activity of a Family 11 Glycoside Hydrolase

TitleStrategies for Modulating the pH-Dependent Activity of a Family 11 Glycoside Hydrolase
Publication TypeJournal Article
Year of Publication2013
AuthorsLudwiczek, ML, D'Angelo, I, Yalloway, GN, Brockerman, JA, Okon, M, Nielsen, JE, Strynadka, NCJ, Withers, SG, McIntosh, LP
JournalBIOCHEMISTRY
Volume52
Pagination3138-3156
Date PublishedMAY 7
ISSN0006-2960
Abstract

The pH-dependent activity of wild-type Bacillus circulans xylanase (BcX) is set by the pK(a) values of its nucleophile Glu78 and general acid/base Glu172. Herein, we examined several strategies to manipulate these pK(a) values and thereby shift the pH(opt) at which BcX is optimally active. Altering the global charge of BcX through random succinylation had no significant effect. Mutation of residues near or within the active site of BcX, but not directly contacting the catalytic carboxyls, either had little effect or reduced its pH(opt), primarily by lowering the apparent pKa value of Glu78. However, mutations causing the largest pK(a) changes also impaired activity. Although not found as a general acid/base in naturally occurring xylanases, substitution of Glu172 with a His lowered the pH(opt) of BcX from 5.6 to 4.7 while retaining 8% activity toward a xylobioside substrate. Mutation of Asn35, which contacts Glu172, to either His or Glu also led to a reduction in pH(opt) by similar to 1.2 units. Detailed pKa measurements by NMR spectroscopy revealed that, despite the opposite charges of the introduced residues, both the N35H and N35E forms of BcX utilize a reverse protonation mechanism. In this mechanism, the pK(a) value of the general acid is lower than that of the nucleophile, and only a small population of enzyme is in a catalytically competent ionization state. However, overall activity is maintained due to the increased strength of the general acid. This study illustrates several routes for altering the pH-dependent properties of xylanases, while also providing valuable insights into complex protein electrostatics.

DOI10.1021/bi400034m