Research & Faculty

Default Header Image

SITE-DIRECTED MUTATION OF THE PUTATIVE CATALYTIC RESIDUES OF ENDOGLUCANASE CENA FROM CELLULOMONAS-FUMI

TitleSITE-DIRECTED MUTATION OF THE PUTATIVE CATALYTIC RESIDUES OF ENDOGLUCANASE CENA FROM CELLULOMONAS-FUMI
Publication TypeJournal Article
Year of Publication1995
AuthorsDamude, HG, Withers, SG, KILBURN, DG, MILLER, RC, WARREN, RAJ
JournalBIOCHEMISTRY
Volume34
Pagination2220-2224
Date PublishedFEB 21
ISSN0006-2960
Abstract

The catalytic domains of beta-1,4-glucanases can be grouped into families of related amino acid sequences. The endoglucanase CenA from Cellulomonas fimi is a member of family B. All enzymes from this family are believed to hydrolyze beta-1,4-glucosidic bonds using a general acid-base catalytic mechanism resulting in inversion of anomeric configuration at the scissile bond. Three-dimensional structures for two cellulases from family B have been determined by X-ray crystallographic analysis. These structures show that there are four Asp residues which are in a position to function as acid catalyst, base catalyst, and/or transition state stabilizers. These aspartates are conserved in all members of family B. The roles of Asp216, Asp252, Asp287, and Asp392, the corresponding amino acids in CenA, were determined. These aspartates have been systematically replaced with alanine and glutamate via site-directed mutagenesis, and the resulting effect on activity, substrate specificity, and overall structure has been determined. Changes in overall structure were monitored using circular dichroism spectroscopy, and no significant differences between the wild-type and mutant proteins were found. Active site structure was also found to be intact as all proteins bound to a cellobiose affinity column, The Michaelis-Menten parameters of the enzyme were determined on 2,4-dinitrophenyl cellobioside as well as (carboxymethyl)cellulose and phosphoric acid-swollen cellulose. Initial characterization of mutant proteins indicates that Asp252 and Asp392 are the acid and base catalysts, respectively, in CenA. Residue Asp287 appears to aid Asp252 in acid catalysis, and Asp216 is not absolutely required for catalysis.

DOI10.1021/bi00007a016