Title | The separation of dimethylarsinic acid, methylarsonous acid, methylarsonic acid, arsenate and dimethylarsinous acid on the Hamilton PRP-X100 anion-exchange column |
Publication Type | Journal Article |
Year of Publication | 1999 |
Authors | Gailer, J, Madden, S, Cullen, WR, Denton, MB |
Journal | Applied Organometallic Chemistry |
Volume | 13 |
Pagination | 837-843 |
Date Published | Nov |
Type of Article | Article |
ISBN Number | 0268-2605 |
Keywords | ARSENATE, ARSENITE, BEHAVIOR, DIMETHYLARSINIC ACID, dimethylarsinous acid, ENVIRONMENT, ENZYMATIC METHYLATION, GLUTATHIONE, HPLC-ICP-AES, LAKE-BIWA, METABOLISM, METHYLARSENICALS, methylarsonic acid, methylarsonous acid, SPECIATION, SPECTROMETRY |
Abstract | In order to separate the potential arsenite metabolites methylarsonous acid and dimethylarsinous acid from arsenite, arsenate, methylarsonic acid and dimethylarsinic acid, the pH-dependent retention behaviour of all six arsenic compounds was studied on a Hamilton PRP-X100 anion-exchange column with 30 mM phosphate buffers (pH 5, 6, 7, 8 and 9) containing 20% (v/v) methanol as mobile phase and employing an inductively coupled plasma atomic emission spectrometer (ICP-AES) as the arsenic-specific detector. Baseline separation of dimethylarsinic acid, methylarsonous acid, methylarsonic acid, arsenate and dimethylarsinous acid was achieved with a 30 mmol dm(-3) phosphate buffer (pH 5)-methanol mixture (80:20, v/v) in 25 min. Arsenite is not baseline-separated from dimethylarsinic acid under these conditions, Copyright (C) 1999 John Wiley & Sons, Ltd. |
URL | <Go to ISI>://000083654200005 |
