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Recent Developments in Glycoside Synthesis with Glycosynthases and Thioglycoligases

TitleRecent Developments in Glycoside Synthesis with Glycosynthases and Thioglycoligases
Publication TypeJournal Article
Year of Publication2009
AuthorsRakic, B, Withers, SG
JournalAUSTRALIAN JOURNAL OF CHEMISTRY
Volume62
Pagination510-520
ISSN0004-9425
Abstract

Glycosynthases are hydrolytically incompetent engineered glycosidases that catalyze the high-yielding synthesis of glycoconjugates from glycosyl fluoride donor substrates and appropriate acceptors. Glycosynthases from more than 10 glycoside hydrolase families have now been generated, allowing the synthesis of a wide range of oligosaccharides. Recent examples include glycosynthase-mediated syntheses of xylo-oligosaccharides, xyloglucans, glycolipids, and aryl glycosides. Glycosynthases have also now been generated from inverting glycosidases, increasing the range of enzyme scaffolds. Improvement of glycosynthase activity and broadening of specificity has been achieved through directed evolution approaches, and several novel high-throughput screens have been developed to allow this. Finally, metabolically stable glycoside analogues have been generated using another class of mutant glycosidases: thioglycoligases. Recent developments in all these aspects are discussed.

DOI10.1071/CH09059