Title | Pulling Genetic RNA out of Tobacco Mosaic Virus Using Single-Molecule Force Spectroscopy |
Publication Type | Journal Article |
Year of Publication | 2010 |
Authors | Liu, N, Peng, B, Lin, Y, Su, Z, Niu, Z, Wang, Q, Zhang, W, Li, H, Shen, J |
Journal | JOURNAL OF THE AMERICAN CHEMICAL SOCIETY |
Volume | 132 |
Pagination | 11036-11038 |
Date Published | AUG 18 |
ISSN | 0002-7863 |
Abstract | RNA-coat protein interactions in intact tobacco mosaic virus have been investigated for the first time directly on the single-molecule level by pulling the genetic RNA step by step out of the helical groove formed by its protein coat. The effects of pulling speed and pH on RNA-protein interactions are presented. In addition, the rebinding behavior of the detached RNA with the protein coat is discussed. Our results demonstrate the possibility of studying nucleic acid-protein interactions in more complicated systems using AFM-based single-molecule force spectroscopy. |
DOI | 10.1021/ja1052544 |
