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Pulling Genetic RNA out of Tobacco Mosaic Virus Using Single-Molecule Force Spectroscopy

TitlePulling Genetic RNA out of Tobacco Mosaic Virus Using Single-Molecule Force Spectroscopy
Publication TypeJournal Article
Year of Publication2010
AuthorsLiu, N, Peng, B, Lin, Y, Su, Z, Niu, Z, Wang, Q, Zhang, W, Li, H, Shen, J
JournalJOURNAL OF THE AMERICAN CHEMICAL SOCIETY
Volume132
Pagination11036-11038
Date PublishedAUG 18
ISSN0002-7863
Abstract

RNA-coat protein interactions in intact tobacco mosaic virus have been investigated for the first time directly on the single-molecule level by pulling the genetic RNA step by step out of the helical groove formed by its protein coat. The effects of pulling speed and pH on RNA-protein interactions are presented. In addition, the rebinding behavior of the detached RNA with the protein coat is discussed. Our results demonstrate the possibility of studying nucleic acid-protein interactions in more complicated systems using AFM-based single-molecule force spectroscopy.

DOI10.1021/ja1052544