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Nuclear Magnetic Resonance Spectral Assignments of alpha-1,4-Galactosyltransferase LgtC from Neisseria meningitidis: Substrate Binding and Multiple Conformational States

TitleNuclear Magnetic Resonance Spectral Assignments of alpha-1,4-Galactosyltransferase LgtC from Neisseria meningitidis: Substrate Binding and Multiple Conformational States
Publication TypeJournal Article
Year of Publication2012
AuthorsChan, PHW, Weissbach, S, Okon, M, Withers, SG, McIntosh, LP
JournalBIOCHEMISTRY
Volume51
Pagination8278-8292
Date PublishedOCT 16
ISSN0006-2960
Abstract

Lipopolysaccharide alpha-1,4-galactosyltransferase C (LgtC) from Neisseria meningitidis is responsible for a key step in lipooligosaccharide biosynthesis involving the transfer of alpha-galactose from the sugar donor UDPgalactose to a terminal acceptor lactose. Crystal structures of the complexes of LgtC with Mn2+ and the sugar donor analogue UDP-2-deoxy-2-fluorogalactose in the absence and presence of the sugar acceptor analogue 4'-deoxylactose provided key insights into the galactosyl-transfer mechanism. Combined with kinetic analyses, the enzymatic mechanism of LgtC appears to involve a ``front-side attack{''} S(N)i-like mechanism with a short-lived oxocarbenium-phosphate ion pair intermediate. As a prerequisite for investigating the required roles of structural dynamics in this catalytic mechanism by nuclear magnetic resonance techniques, the transverse relaxation-optimized amide N-15 heteronuclear single-quantum correlation and methyl C-13 heteronuclear multiple-quantum correlation spectra of LgtC in its apo, substrate analogue, and product complexes were partially assigned. This was accomplished using a suite of complementary spectroscopic approaches, combined with selective isotopic labeling and mutagenesis of all the isoleucine residues in the protein. Only similar to 70% of the amide signals could be detected, whereas more than the expected number of methyl signals were observed, indicating that LgtC adopts multiple interconverting conformational states. Chemical shift perturbation mapping provided insights into substrate and product binding, including the demonstration that the sugar donor analogue (UDP-2FGal) associates with LgtC only in the presence of a metal ion (Mg2+). These spectral assignments provide the foundation for detailed studies of the conformational dynamics of LgtC.

DOI10.1021/bi3010279