Title | Glycosphingolipid synthesis employing a combination of recombinant glycosyltransferases and an endoglycoceramidase glycosynthase |
Publication Type | Journal Article |
Year of Publication | 2011 |
Authors | Rich, JR, Cunningham, A-M, Gilbert, M, Withers, SG |
Journal | CHEMICAL COMMUNICATIONS |
Volume | 47 |
Pagination | 10806-10808 |
ISSN | 1359-7345 |
Abstract | Glycosynthase mutants of Rhodococcus sp. endo-glycoceramidase II efficiently synthesize complex glycosphingolipids. Glycosyl fluoride donors may be assembled via sequential glycosyltransferase-catalysed glycosylation of lactosyl fluoride. Alternatively, lactosyl fluoride may be coupled to sphingosine prior to subsequent glycosylation steps. |
DOI | 10.1039/c1cc13885e |
