Research & Faculty

Default Header Image

Glycosphingolipid synthesis employing a combination of recombinant glycosyltransferases and an endoglycoceramidase glycosynthase

TitleGlycosphingolipid synthesis employing a combination of recombinant glycosyltransferases and an endoglycoceramidase glycosynthase
Publication TypeJournal Article
Year of Publication2011
AuthorsRich, JR, Cunningham, A-M, Gilbert, M, Withers, SG
JournalCHEMICAL COMMUNICATIONS
Volume47
Pagination10806-10808
ISSN1359-7345
Abstract

Glycosynthase mutants of Rhodococcus sp. endo-glycoceramidase II efficiently synthesize complex glycosphingolipids. Glycosyl fluoride donors may be assembled via sequential glycosyltransferase-catalysed glycosylation of lactosyl fluoride. Alternatively, lactosyl fluoride may be coupled to sphingosine prior to subsequent glycosylation steps.

DOI10.1039/c1cc13885e