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Fine tuning of beta-glucosidase inhibitory activity in the 2,5-dideoxy-2,5-imino-D-mannitol (DMDP) system

TitleFine tuning of beta-glucosidase inhibitory activity in the 2,5-dideoxy-2,5-imino-D-mannitol (DMDP) system
Publication TypeJournal Article
Year of Publication2006
AuthorsWrodnigg, TM, Stuetz, AE, Tarling, CA, Withers, SG
JournalCARBOHYDRATE RESEARCH
Volume341
Pagination1717-1722
Date PublishedJUL 24
ISSN0008-6215
Abstract

Based on our extensive studies of D-glucosidase inhibiting 2,5-dideoxy-2,5-imino-D-mannitol derivatives, we have been trying to create a series of fluorescent derivatives with a view to an `inhibitory activity ruler' based on competitive displacement reactions of non-fluorescent inhibitors by fluorescent ones and vice versa, which can be performed and followed in microtiter plates or on-chips. Thus, a set of compounds was assembled with K-i values between 2 nM and 1 mu M against Agrobacterium sp. beta-glucosidase. (c) 2006 Elsevier Ltd. All rights reserved.

DOI10.1016/j.carres.2006.03.010