|Title||Fine tuning of beta-glucosidase inhibitory activity in the 2,5-dideoxy-2,5-imino-D-mannitol (DMDP) system|
|Publication Type||Journal Article|
|Year of Publication||2006|
|Authors||Wrodnigg, TM, Stuetz, AE, Tarling, CA, Withers, SG|
|Date Published||JUL 24|
Based on our extensive studies of D-glucosidase inhibiting 2,5-dideoxy-2,5-imino-D-mannitol derivatives, we have been trying to create a series of fluorescent derivatives with a view to an `inhibitory activity ruler' based on competitive displacement reactions of non-fluorescent inhibitors by fluorescent ones and vice versa, which can be performed and followed in microtiter plates or on-chips. Thus, a set of compounds was assembled with K-i values between 2 nM and 1 mu M against Agrobacterium sp. beta-glucosidase. (c) 2006 Elsevier Ltd. All rights reserved.