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Synthesis and characterisation of novel chromogenic substrates for human pancreatic alpha-amylase

TitleSynthesis and characterisation of novel chromogenic substrates for human pancreatic alpha-amylase
Publication TypeJournal Article
Year of Publication2004
AuthorsDamager, I, Numao, S, Chen, HM, BRAYER, GD, Withers, SG
JournalCARBOHYDRATE RESEARCH
Volume339
Pagination1727-1737
Date PublishedJUL 12
ISSN0008-6215
Abstract

Derivatives of maltose and maltotriose were chemically synthesised as substrates for human pancreatic alpha-amylases and subjected to kinetic analysis. Rates measured were shown to reflect both hydrolysis and transglycosylation reactions. 4-O-Methylated derivatives of these substrates underwent only hydrolysis, thereby simplifying kinetic analyses. These modified substrates may be used for the detection and kinetic analysis of a-amylases, and are useful in rapidly screening for novel a-amylase inhibitors and for subsequent kinetic characterisation. (C) 2004 Published by Elsevier Ltd.

DOI10.1016/j.carres.2004.05.019