| Title | Synthesis and characterisation of novel chromogenic substrates for human pancreatic alpha-amylase |
| Publication Type | Journal Article |
| Year of Publication | 2004 |
| Authors | Damager, I, Numao, S, Chen, HM, BRAYER, GD, Withers, SG |
| Journal | CARBOHYDRATE RESEARCH |
| Volume | 339 |
| Pagination | 1727-1737 |
| Date Published | JUL 12 |
| ISSN | 0008-6215 |
| Abstract | Derivatives of maltose and maltotriose were chemically synthesised as substrates for human pancreatic alpha-amylases and subjected to kinetic analysis. Rates measured were shown to reflect both hydrolysis and transglycosylation reactions. 4-O-Methylated derivatives of these substrates underwent only hydrolysis, thereby simplifying kinetic analyses. These modified substrates may be used for the detection and kinetic analysis of a-amylases, and are useful in rapidly screening for novel a-amylase inhibitors and for subsequent kinetic characterisation. (C) 2004 Published by Elsevier Ltd. |
| DOI | 10.1016/j.carres.2004.05.019 |
