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Novel catalytic mechanism of glycoside hydrolysis based on the structure of an NAD(+)/Mn(2+)-dependent phospho-alpha-glucosidase from Bacillus subtilis

TitleNovel catalytic mechanism of glycoside hydrolysis based on the structure of an NAD(+)/Mn(2+)-dependent phospho-alpha-glucosidase from Bacillus subtilis
Publication TypeJournal Article
Year of Publication2004
AuthorsRajan, SS, Yang, XJ, Collart, F, Yip, VLY, Withers, SG, Varrot, A, Thompson, J, Davies, GJ, Anderson, WF
JournalSTRUCTURE
Volume12
Pagination1619-1629
Date PublishedSEP
ISSN0969-2126
Abstract

GlvA, a 6-phospho-alpha-glucosidase from Bacillus subtilis, catalyzes the hydrolysis of maltose-6'-phosphate and belongs to glycoside hydrolase family GH4. GH4 enzymes are unique in their requirement for NAD(H) and a divalent metal for activity. We have determined the crystal structure of GlvA in complex with its ligands to 2.05 Angstrom resolution. Analyses of the active site architecture, in conjunction with mechanistic studies and precedent from the nucleotide diphosphate hexose dehydratases and other systems, suggest a novel mechanism of glycoside hydrolysis by GlvA that involves both the NAD(H) and the metal.

DOI10.1016/j.str.2004.06.020