Research & Teaching Faculty

Default Header Image

Identification of the catalytic nucleophile of endoglucanase I from Fusarium oxysporum by mass spectrometry

TitleIdentification of the catalytic nucleophile of endoglucanase I from Fusarium oxysporum by mass spectrometry
Publication TypeJournal Article
Year of Publication1997
AuthorsMacKenzie, LF, Davies, GJ, Schulein, M, Withers, SG
JournalBIOCHEMISTRY
Volume36
Pagination5893-5901
Date PublishedMAY 13
ISSN0006-2960
Abstract

{The endoglucanase EG I from Fusarium oxysporum catalyzes the hydrolysis of cellulose via a double-displacement mechanism involving the formation and hydrolysis of a glycosyl-enzyme intermediate. Treatment of EG I with 2',4'-dinitrophenyl-2-deoxy-2-fluoro-beta-D-cellobioside results in the time-dependent inactivation of the enzyme (k(i) = 1.36 min(-1)

DOI10.1021/bi962962h