Research & Teaching Faculty

Default Header Image

IDENTIFICATION OF DERIVATIZED PEPTIDES WITHOUT RADIOLABELS - TANDEM MASS-SPECTROMETRIC LOCALIZATION OF THE TAGGED ACTIVE-SITE NUCLEOPHILES OF 2 CELLULASES AND A BETA-GLUCOSIDASE

TitleIDENTIFICATION OF DERIVATIZED PEPTIDES WITHOUT RADIOLABELS - TANDEM MASS-SPECTROMETRIC LOCALIZATION OF THE TAGGED ACTIVE-SITE NUCLEOPHILES OF 2 CELLULASES AND A BETA-GLUCOSIDASE
Publication TypeJournal Article
Year of Publication1995
AuthorsTULL, D, MIAO, SC, Withers, SG, AEBERSOLD, R
JournalANALYTICAL BIOCHEMISTRY
Volume224
Pagination509-514
Date PublishedJAN 20
ISSN0003-2697
Abstract

A new method that uses nonradioactive active site-directed enzyme inactivators and high-performance liquid chromatography-electrospray ionization tandem mass spectrometry (HPLC-ESLMS/MS) to identify labeled peptides in a proteolytic digest is described, This method relies upon the fragmentation of labeled peptides in a predictable and reproducible manner in the collision cell. of a tandem mass spectrometer, The exoglycanase from Cellulomonas fimi, endoglucanase C from Clostridium thermocellum, and the beta-glucosidase from Agrobacterium faecalis were labeled using 2-deoxy-2-halo-beta-glycosides, digested with pepsin, and subjected to HPLC-ESIMS/MS analysis, scanning in the neutral loss mode, Under these conditions only peptides that lose the (known) mass of the label are detected, Preliminary identification of candidate peptides can be achieved from the mass measured, in combination with the known sequence of the protein, Peptide identity can be confirmed through subsequent sequencing, either via further tandem MS experiments or via the Edman degradation, In all cafes the peptides identified in this manner were consistent with those identified by the standard radioactive method, This mass spectrometric method represents a rapid, nonradioisotopic solution to the problem of identifying a modified peptide in a complex mixture, The technique is also sensitive, requiring only picomole amounts of protein, (C) 1995 Academic Press,Inc.

DOI10.1006/abio.1995.1080