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An NMR approach to structural proteomics

TitleAn NMR approach to structural proteomics
Publication TypeJournal Article
Year of Publication2002
AuthorsYee, A, Chang, XQ, Pineda-Lucena, A, Wu, B, Semesi, A, Le, B, Ramelot, T, Lee, GM, Bhattacharyya, S, Gutierrez, P, Denisov, A, Lee, CH, Cort, JR, Kozlov, G, Liao, J, Finak, G, Chen, L, Wishart, D, Lee, W, McIntosh, LP, Gehring, K, Kennedy, MA, Edwards, AM, Arrowsmith, CH
JournalProceedings of the National Academy of Sciences of the United States of America
Volume99
Pagination1825-1830
Date PublishedFeb
Type of ArticleArticle
ISBN Number0027-8424
KeywordsASSIGNMENTS, CATH, DOMAIN, PROGRAM, PROTEINS, SPECTRA
Abstract

The influx of genomic sequence information has led to the concept of structural proteomics, the determination of protein structures on a genome-wide scale. Here we describe an approach to structural proteomics of small proteins using NMR spectroscopy. Over 500 small proteins from several organisms were cloned, expressed, purified, and evaluated by NMIR. Although there was variability among proteomes, overall 20% of these proteins were found to be readily amenable to NMR structure determination. NMIR sample preparation was centralized in one facility, and a distributive approach was used for NMR data collection and analysis. Twelve structures are reported here as part of this approach, which allowed us to infer putative functions for several conserved hypothetical proteins.

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