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New Tools for Life Science Research Based on Fiber-Optic-Linked Raman and Resonance Raman Spectroscopy

TitleNew Tools for Life Science Research Based on Fiber-Optic-Linked Raman and Resonance Raman Spectroscopy
Publication TypeBook Chapter
Year of Publication2007
AuthorsBlades, MW, Schulze, HG, Konorov, SO, Addison, CJ, Jirasek, AI, Turner, RFB
EditorKneipp, K, Aroca, R, Kneipp, H, E. trupByrne, W
Book TitleNew Approaches in Biomedical Spectroscopy
Series TitleAcs Symposium Series
Volume963
Pagination1-13
PublisherAmer Chemical Soc
CityWashington
ISBN Number0097-6156978-0-8412-7437-2
Keywords1, 2-DIOXYGENASE, PHOTONIC-CRYSTAL FIBER, PICOSECOND PULSES
Abstract

Fiber-optic probes can exploit a favorable excitation radiation distribution within the sample that allows the use of higher laser power levels which, in turn, can yield a higher signal-to-noise ratio (SNR) for a given experiment without increasing the risk of analyte photo-damage. We have developed specialized fiber-optic probes for ultraviolet resonance Raman spectroscopy (UVRRS) that offer several advantages over conventional excitation/collection methods used for UVRRS. These probes are ideally suited for UVRRS studies involving biopolymers and small bio-molecules, in both native (e.g. physiological) and non-native (e.g. anoxic) solution environments. We have also developed novel probes based on hollow-core photonic band-gap fibers that virtually eliminate the generation of silica Raman scattering within the excitation fiber which often limits the utility of fiber-optic Raman probes in turbid media or near surfaces. These probes may offer advantages for some biomedical applications.

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