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Mechanism of Thermoanaerobacterium saccharolyticum ss-xylosidase: Kinetic studies

TitleMechanism of Thermoanaerobacterium saccharolyticum ss-xylosidase: Kinetic studies
Publication TypeJournal Article
Year of Publication2002
AuthorsVocadlo, DJ, Wicki, J, RUPITZ, K, Withers, SG
JournalBIOCHEMISTRY
Volume41
Pagination9727-9735
Date PublishedAUG 6
ISSN0006-2960
Abstract

{The catalytic mechanism of Thermoanaerobacterium saccharolyticum beta-xylosidase (XynB) from family 39 of glycoside hydrolases has been subjected to a detailed kinetic investigation using a range of substrates. The enzyme exhibits a bell-shaped pH dependence of k(cat)/K(m), reflecting apparent pK(a) values of 4.1 and 6.8. The k(cat) and k(cat)/K(m) values for a series of aryl xylosides have been measured and used to construct two Bronsted plots. The plot of log(k(cat)/K(m)) against the pK(a) of the leaving group reveals a significant correlation (beta(1g) = -0.97, r(2) = 0.94

DOI10.1021/bi020077v