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Magnetic resonance imaging evaluation of photodynamic therapy-induced hemorrhagic necrosis in the murine M1 tumor model

TitleMagnetic resonance imaging evaluation of photodynamic therapy-induced hemorrhagic necrosis in the murine M1 tumor model
Publication TypeJournal Article
Year of Publication1997
AuthorsWinsborrow, BG, Grondey, H, Savoie, H, Fyfe, CA, Dolphin, D
JournalPhotochemistry and Photobiology
Volume66
Pagination847-852
Date PublishedDec
Type of ArticleArticle
ISBN Number0031-8655
KeywordsMICE, NORMAL BRAIN, SPECTROSCOPY
Abstract

Proton magnetic resonance imaging (MRI) and histological methods were used to evaluate photodynamic therapy (PDT)-induced hemorrhagic necrosis in the murine M1 tumor within 72 h of treatment of male DBA/2 mice, The effects of three photosensitizing drugs were investigated: Photofrin(R) (n = 4), Zn (II) phthalocyanine (n = 7) and benzoporphyrin derivative monoacid ring A (n = 11). As noted in previous studies of PDT using MRI, MRI makes possible serial, noninvasive, in vivo observation of tissue response to PDT. Our serial study of MRI and histological data confirms that tumors responded in the same way to PDT treatment using the three photosensitizing drugs: vascular damage followed by hemorrhagic necrosis. Most importantly and unlike previous MRI studies of PDT, we used a very high field magnet that enhanced the effect of magnetic susceptibility on image signal when blood is processed by the body after PDT-induced hemorrhagic necrosis. This last finding demonstrates the utility of high field magnets and the importance of localized, serial experiments in future magnetic resonance studies of PDT.

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