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The inhibition of glutamate racemase by D-N-hydroxyglutamate

TitleThe inhibition of glutamate racemase by D-N-hydroxyglutamate
Publication TypeJournal Article
Year of Publication1997
AuthorsGlavas, S, Tanner, ME
JournalBioorganic & Medicinal Chemistry Letters
Volume7
Pagination2265-2270
Date PublishedSep
Type of ArticleArticle
ISBN Number0960-894X
KeywordsCLONING, DIAMINOPIMELIC ACID, EPIMERASE, ESCHERICHIA-COLI, GENE, L-ALANINE, LACTOBACILLUS, MURI, PEDIOCOCCUS-PENTOSACEUS, resistance
Abstract

We report that D-N-hydroxyglutamate is ii competitive inhibitor of glutamate racemase (K-I=56 mu M). The compound acts as an alternate substrate and is converted into a-ketoglutarate and ammonia (K-M=57 mu M, k(cat)/K-M=3.2x10(3) M(-1)sec(-1)), An imine intermediate is likely the species causing the inhibition. L-N-hydroxyglutamate is a weak inhibitor and is slowly converted to alpha-ketoglutarate (k(cat)/K-M=30 M(-1)sec(-1)). (C) 1997 Elsevier Science Ltd.

URL<Go to ISI>://A1997XX50700025