|Title||Glycosynthase-Mediated Assembly of Xylanase Substrates and Inhibitors|
|Publication Type||Journal Article|
|Year of Publication||2011|
|Authors||Goddard-Borger, ED, Fiege, B, Kwan, EM, Withers, SG|
|Date Published||JUL 25|
An exo-beta-xylosidase mutant with glycosynthase activity was created to aid in the synthesis of xylanase substrates and inhibitors. Simple monosaccharides were easily elaborated into di-, tri- and tetrasaccharides by using this enzyme. Some products proved to be surprisingly potent inhibitors of xylanases from glycoside hydrolase families 10 and 11.