| Title | Glycosynthase-Mediated Assembly of Xylanase Substrates and Inhibitors |
| Publication Type | Journal Article |
| Year of Publication | 2011 |
| Authors | Goddard-Borger, ED, Fiege, B, Kwan, EM, Withers, SG |
| Journal | CHEMBIOCHEM |
| Volume | 12 |
| Pagination | 1703-1711 |
| Date Published | JUL 25 |
| ISSN | 1439-4227 |
| Abstract | An exo-beta-xylosidase mutant with glycosynthase activity was created to aid in the synthesis of xylanase substrates and inhibitors. Simple monosaccharides were easily elaborated into di-, tri- and tetrasaccharides by using this enzyme. Some products proved to be surprisingly potent inhibitors of xylanases from glycoside hydrolase families 10 and 11. |
| DOI | 10.1002/cbic.201100229 |
