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Glycosynthase-Mediated Assembly of Xylanase Substrates and Inhibitors

TitleGlycosynthase-Mediated Assembly of Xylanase Substrates and Inhibitors
Publication TypeJournal Article
Year of Publication2011
AuthorsGoddard-Borger, ED, Fiege, B, Kwan, EM, Withers, SG
JournalCHEMBIOCHEM
Volume12
Pagination1703-1711
Date PublishedJUL 25
ISSN1439-4227
Abstract

An exo-beta-xylosidase mutant with glycosynthase activity was created to aid in the synthesis of xylanase substrates and inhibitors. Simple monosaccharides were easily elaborated into di-, tri- and tetrasaccharides by using this enzyme. Some products proved to be surprisingly potent inhibitors of xylanases from glycoside hydrolase families 10 and 11.

DOI10.1002/cbic.201100229