|Publication Type||Journal Article|
|Year of Publication||2000|
|Authors||Rye, CS, Withers, SG|
|Journal||CURRENT OPINION IN CHEMICAL BIOLOGY|
Insights into glycosidase mechanisms have come from X-ray crystallographic studies on complexes with substrate analogs and inhibitors, representing all the intermediate species along the reaction coordinate. Site-directed mutagenesis continues to play a significant role in understanding mechanisms, but is also proving important in generating glycosidases of modified mechanism or specificity.