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A glyco-gold nanoparticle based assay for alpha-2,8-polysialyltransferase from Neisseria meningitidis

TitleA glyco-gold nanoparticle based assay for alpha-2,8-polysialyltransferase from Neisseria meningitidis
Publication TypeJournal Article
Year of Publication2013
AuthorsYu, C-C, De Huang, L-, Kwan, DH, Wakarchuk, WW, Withers, SG, Lin, C-C
JournalCHEMICAL COMMUNICATIONS
Volume49
Pagination10166-10168
ISSN1359-7345
Abstract

We designed a novel strategy for sensitively detecting the activity of alpha-2,8-polysialyltransferase (PST) by a combination of ganglioside GD3 functionalized gold nanoparticles and inactive endosialidase. We anticipate that this new method will facilitate the search for PST inhibitors as well as for improved mutant forms of PST in directed evolution experiments.

DOI10.1039/c3cc45147j