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Fluoro-glycosyl acridinones are ultra-sensitive active site titrating agents for retaining beta-glycosidases

TitleFluoro-glycosyl acridinones are ultra-sensitive active site titrating agents for retaining beta-glycosidases
Publication TypeJournal Article
Year of Publication2014
AuthorsDuo, T, Goddard-Borger, ED, Withers, SG
JournalCHEMICAL COMMUNICATIONS
Volume50
Pagination9379-9382
Date PublishedAUG 25
ISSN1359-7345
Abstract

Novel fluorogenic 2-deoxy-2-fluoroglycosyl acridinone active site titrating reagents were synthesised and kinetic parameters determined for their inactivation of two retaining beta-glucosidases, a beta-galactosidase, a beta-xylosidase and several cellulases. Fluorescence-monitored active site titration using this class of reagents reliably measured active enzyme concentrations down to 3 nM.

DOI10.1039/c4cc03299c