|Title||Fluoro-glycosyl acridinones are ultra-sensitive active site titrating agents for retaining beta-glycosidases|
|Publication Type||Journal Article|
|Year of Publication||2014|
|Authors||Duo, T, Goddard-Borger, ED, Withers, SG|
|Date Published||AUG 25|
Novel fluorogenic 2-deoxy-2-fluoroglycosyl acridinone active site titrating reagents were synthesised and kinetic parameters determined for their inactivation of two retaining beta-glucosidases, a beta-galactosidase, a beta-xylosidase and several cellulases. Fluorescence-monitored active site titration using this class of reagents reliably measured active enzyme concentrations down to 3 nM.