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DISTINGUISHING XYLOGLUCANASE ACTIVITY IN ENDO-beta(1 -> 4)GLUCANASES

TitleDISTINGUISHING XYLOGLUCANASE ACTIVITY IN ENDO-beta(1 -> 4)GLUCANASES
Publication TypeBook Chapter
Year of Publication2012
AuthorsEklof, JM, Ruda, MC, Brumer, H
EditorGilbert, HJ
Book TitleCELLULASES
Series TitleMethods in Enzymology
Volume510
Pagination97-120
ISBN Number978-0-12-415931-0
ISBN0076-6879
Abstract

The ability of beta-glucanases to cleave xyloglucans, a family of highly decorated beta-glucans ubiquitous in plant biomass, has traditionally been overlooked in functional biochemical studies. An emerging body of data indicates, however, that a spectrum of xyloglucan specificity resides in diverse glycoside hydrolases from a range of carbohydrate-active enzyme families including classic ``cellulase{''} families. This chapter outlines a series of enzyme kinetic and product analysis methods to establish degrees of xyloglucan specificity and modes of action of glycosidases emerging from enzyme discovery projects.

DOI10.1016/B978-0-12-415931-0.00006-9