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Current approaches for global post-translational modification discovery and mass spectrometric analysis

TitleCurrent approaches for global post-translational modification discovery and mass spectrometric analysis
Publication TypeJournal Article
Year of Publication2008
AuthorsHoffman, MD, Sniatynski, MJ, Kast, J
JournalAnalytica Chimica Acta
Volume627
Pagination50-61
Date PublishedOct
Type of ArticleReview
ISBN Number0003-2670
Keywordscomputational, glycosylation, IDENTIFICATION, IONIZATION, marker ion, MASS SPECTROMETRY, MODIFIED PEPTIDES, neutral loss, post-translational modifications, protein array, PROTEIN-PHOSPHORYLATION, PROTEOMIC ANALYSIS, S-NITROSYLATION, SEQUENCES, SIGNALING NETWORKS, SPECTRA
Abstract

More and more attention is being focused on the analysis of post-translational modifications (PTMs) on proteins as researchers are continually learning how essential they are for proper cellular function. As there are hundreds of different types of known PTMs, traditional methods of modification analysis are incapable of comprehensively monitoring for post-translational modifications, a task which is a necessity for truly understanding a cell’s biology. This review highlights recent developments in novel multiplexed methods of PTM analysis including: fluorescent stain and immuno-based methods, hardware-based mass spectrometric methods and computational-based mass spectrometric methods. Many of these techniques show great promise and will likely be a valuable resource for the biological community. (C) 2008 Elsevier B.V. All rights reserved.
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