Course Level:
Graduate Level
Academic Year:
2016/2017
Instructor: Juergen Kast
Office hours: by appointment
Textbook: none
Course outline:
- Introduction: mass spectrometry and life sciences applications
- MALDI and electrospray ion sources
- Mass analyzers: quadrupole mass filters, quadrupole ion traps, time-of-flight, ion cyclotron resonance, Orbitraps
- MS applications: protein folding, reaction kinetics, non-covalent (macromolecular) complexes, tissue profiling, tissue imaging
- Tandem mass spectrometry: fragmentation approaches, fragmentation on different mass analyzers
- Tandem mass spectrometers: triple quadrupoles, quadrupole ion traps, Q-TOFs, TOF-TOFs
- MS/MS applications: peptide sequencing, post-translational modifications, protein cross-linking, drug/metabolite analysis, stable isotope labeling