Abstract:
We design and develop a range of functional nanoparticles and molecular recognition and transduction protocols; I will introduce some of our work on capacitative transduction before moving onto new developments in amperometric sensing. This will include the utilization of AC field-assisted microfluidic target capture at nanoparticles prior to presentation at sensors. I will present an example of the ultrasensitive (cancer progression indicative) anti-p53 antibody quantification using antigen-mimicking and signal-generating nanoparticles. Finally, it is clear that an effective intervention in neurodegeneration will require facile pre-symptomatic diagnosis. We have developed a toolbox where nanoparticles isolate neuronal exosomes. The amplified shotgun assaying of the synuclein load of these enables the pre-symptomatic identification of Parkinson’s risk in patients years before current diagnosis.