| Title | Artificial nucleases |
| Publication Type | Journal Article |
| Year of Publication | 2001 |
| Authors | Chen, CHB, Milne, L, Landgraf, R, Perrin, DM, Sigman, DS |
| Journal | Chembiochem |
| Volume | 2 |
| Pagination | 735-740 |
| Date Published | Oct |
| Type of Article | Article |
| ISBN Number | 1439-4227 |
| Keywords | 1, 10-PHENANTHROLINE-COPPER ION, chemical, COLI RNA-POLYMERASE, COMPLEX, copper, DNA, DNA cleavage, DNA recognition, ESCHERICHIA-COLI, NUCLEASE, nucleic acids, OLIGONUCLEOTIDES, PROTEIN, SITE-SPECIFIC NUCLEASE, TRANSCRIPTION INITIATION |
| Abstract | The oxidation of DNA and RNA provides a facile approach for investigating the interaction of nucleic acids wit proteins and oligonucleotides In this article, we have outlined our understanding of the mechanism of DNA scission by 1,10-phenanthroline-copper(I) in the presence of hydrogen peroxide. We also discuss results obtained by using 1,10-phenanthroline - oligonucleotide conjugates in probing the size of the transcriptionally active open complex. Finally, we outline an effective method for converting DNA-binding proteins into site-specific modification agents by using 1,10-phenanthroline-copper(I). |
| URL | <Go to ISI>://000171410000003 |
