|Publication Type||Journal Article|
|Year of Publication||2001|
|Authors||Chen, CHB, Milne, L, Landgraf, R, Perrin, DM, Sigman, DS|
|Type of Article||Article|
|Keywords||1, 10-PHENANTHROLINE-COPPER ION, chemical, COLI RNA-POLYMERASE, COMPLEX, copper, DNA, DNA cleavage, DNA recognition, ESCHERICHIA-COLI, NUCLEASE, nucleic acids, OLIGONUCLEOTIDES, PROTEIN, SITE-SPECIFIC NUCLEASE, TRANSCRIPTION INITIATION|
The oxidation of DNA and RNA provides a facile approach for investigating the interaction of nucleic acids wit proteins and oligonucleotides In this article, we have outlined our understanding of the mechanism of DNA scission by 1,10-phenanthroline-copper(I) in the presence of hydrogen peroxide. We also discuss results obtained by using 1,10-phenanthroline - oligonucleotide conjugates in probing the size of the transcriptionally active open complex. Finally, we outline an effective method for converting DNA-binding proteins into site-specific modification agents by using 1,10-phenanthroline-copper(I).
|URL||<Go to ISI>://000171410000003|