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APPROACHES TO LABELING AND IDENTIFICATION OF ACTIVE-SITE RESIDUES IN GLYCOSIDASES

TitleAPPROACHES TO LABELING AND IDENTIFICATION OF ACTIVE-SITE RESIDUES IN GLYCOSIDASES
Publication TypeJournal Article
Year of Publication1995
AuthorsWithers, SG, AEBERSOLD, R
JournalPROTEIN SCIENCE
Volume4
Pagination361-372
Date PublishedMAR
ISSN0961-8368
Abstract

Glycosidases play a key role in a number of biological processes and, as such, are of considerable clinical and biotechnological importance. Knowledge of the identities of catalytically important active site residues is essential for understanding the catalytic mechanism, for enzyme classification, and for targeted bioengineering of glycosidases with altered characteristics. Here we review and discuss traditional strategies and novel approaches based on tandem mass spectrometry for the identification of the key active site residues in glycosidases.