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C-14-Labeled microcystin-LR administered to Atlantic salmon via intraperitoneal injection provides in vivo evidence for covalent binding of microcystin-LR in salmon livers

TitleC-14-Labeled microcystin-LR administered to Atlantic salmon via intraperitoneal injection provides in vivo evidence for covalent binding of microcystin-LR in salmon livers
Publication TypeJournal Article
Year of Publication1997
AuthorsWilliams, DE, Craig, M, Dawe, SC, Kent, ML, Andersen, RJ, Holmes, CFB
JournalToxicon
Volume35
Pagination985-989
Date PublishedJun
Type of ArticleArticle
ISBN Number0041-0101
Keywordsbiotransformation, CHROMATOGRAPHY, DIARRHETIC SHELLFISH TOXINS, DISEASE, IDENTIFICATION, INHIBITOR, POTENT, PROTEIN PHOSPHATASE, TISSUE DISTRIBUTION, TYPE-1
Abstract

The tissue distribution and clearance of radiolabeled microcystin-LR administered to Atlantic salmon via i.p. injection has been re-examined using uniformly C-14-labeled toxin, Significant differences were found to exist between these results and those obtained when fish received an i.p. injection of tritium-labeled dihydromicrocystin-LR. In addition, MeOH liver extracts were assayed by both phosphatase assay and C-14 counts and the results compared with the total levels of incorporation determined by digestion and subsequent C-14 counting of the same liver tissues. An attempt to investigate the metabolism and to document the putative products was also undertaken. It was found that microcystin-LR was extensively metabolized to compounds that are more polar than the parent compound. (C) 1997 Elsevier Science Ltd.

URL<Go to ISI>://A1997XG73100017