@article {3763,
	title = {Driving forces for DNA adsorption to silica in perchlorate solutions},
	journal = {Journal of Colloid and Interface Science},
	volume = {181},
	number = {2},
	year = {1996},
	note = {ISI Document Delivery No.: UZ536Times Cited: 112Cited Reference Count: 43},
	month = {Aug},
	pages = {635-644},
	type = {Article},
	abstract = {The adsorption of both plasmid and chromosomal duplex DNA to silica is investigated with the aim of determining the dominant forces involved in the binding reaction. Changes in the initial slopes and plateau values of adsorption isotherms for DNA on microcrystalline silica particles are used to establish the sensitivity of the binding reaction to ionic strength, temperature and pH, and DNA size and conformation. Binding is driven by an increase in entropy, with little or no enthalpic contribution. Adsorption isotherm results indicate that three effects, namely: (i) shielded intermolecular electrostatic forces, (ii) dehydration of the DNA and silica surfaces, and (iii) intermolecular hydrogen bond formation in the DNA-silica contact layer, make the dominant contributions to the overall driving force for adsorption. (C) 1996 Academic Press, Inc.},
	keywords = {adsorption isotherms, ARRAYS, CHIPS, CONFORMATION, DNA, DNA adsorption, DNA binding, DNA PURIFICATION, DNA-silica interactions, ELECTROPHORESIS, GLOBULAR-PROTEINS, PLASMID DNA, PURIFICATION, SURFACES, TRANSITION},
	isbn = {0021-9797},
	url = {<Go to ISI>://A1996UZ53600031},
	author = {Melzak, K. A. and Sherwood, C. S. and Turner, R. F. B. and Haynes, C. A.}
}
@article {3357,
	title = {CHARACTERIZATION OF AN OLIGONUCLEOTIDE-BINDING FLUORESCENT LIGAND FOR APPLICATION IN AFFINITY PURIFICATION OF DSDNA},
	journal = {Biotechnology and Bioengineering},
	volume = {48},
	number = {1},
	year = {1995},
	note = {ISI Document Delivery No.: RU627Times Cited: 1Cited Reference Count: 39},
	month = {Oct},
	pages = {25-35},
	type = {Article},
	abstract = {The fluorescent probe PO-PRO-3 was investigated as a potential ligand for the affinity immobilization and purification of genomic or plasmid DNA fragments. Affinities and mechanisms for PO-PRO-3 binding to superhelical and linearized pUC 18 plasmid DNA were examined through measurement of binding isotherms, continuous-variation analysis, and DNA titrations. In addition, the effects of DNA conformation, protein and RNA contaminants, solvent polarity, and ionic strength are examined with the aim of optimizing binding and elution conditions and of assigning limits to the range of applicability of the affinity purification. (C) 1995 John Wiley \& Sons, Inc.},
	keywords = {ANTHRACYCLINE, antibiotics, BIO-POLYMERS, DNA AMPLIFICATION, DNA PURIFICATION, DNA-BINDING FLUOROPHORE, DYES, FLUORESCENT LIGAND, POLYMERASE CHAIN-REACTION, SENSITIVITY},
	isbn = {0006-3592},
	url = {<Go to ISI>://A1995RU62700004},
	author = {Haynes, C. A. and Sherwood, C. S. and Turner, R. F. B.}
}
