@article {TPJ:TPJ13294,
	title = {The composition of surface wax on trichomes of Arabidopsis thaliana differs from wax on other epidermal cells},
	journal = {The Plant Journal},
	year = {2016},
	pages = {n/a{\textendash}n/a},
	keywords = {Arabidopsis thaliana, cuticle, Epidermis, HYDROCARBONS, Pavement cells, trichomes, wax},
	issn = {1365-313X},
	doi = {10.1111/tpj.13294},
	url = {http://dx.doi.org/10.1111/tpj.13294},
	author = {Hegebarth, Daniela and Buschhaus, Christopher and Wu, May and Bird, David and Jetter, Reinhard}
}
@article {1122,
	title = {What do microbes encounter at the plant surface ? Chemical composition of pea leaf cuticular waxes},
	journal = {Plant Physiology},
	volume = {139},
	number = {1},
	year = {2005},
	note = {ISI Document Delivery No.: 962WZTimes Cited: 31Cited Reference Count: 37},
	month = {Sep},
	pages = {519-530},
	type = {Article},
	abstract = {In the cuticular wax mixtures from leaves of pea ( Pisum sativum) cv Avanta, cv Lincoln, and cv Maiperle, more than 70 individual compounds were identified. The adaxial wax was characterized by very high amounts of primary alcohols ( 71\%), while the abaxial wax consisted mainly of alkanes ( 73\%). An aqueous adhesive of gum arabic was employed to selectively sample the epicuticular wax layer on pea leaves and hence to analyze the composition of epicuticular crystals exposed at the outermost surface of leaves. The epicuticular layer was found to contain 74\% and 83\% of the total wax on adaxial and abaxial surfaces, respectively. The platelet-shaped crystals on the adaxial leaf surface consisted of a mixture dominated by hexacosanol, accompanied by substantial amounts of octacosanol and hentriacontane. In contrast, the ribbon-shaped wax crystals on the abaxial surface consisted mainly of hentriacontane (63\%), with approximately 5\% each of hexacosanol and octacosanol being present. Based on this detailed chemical analysis of the wax exposed at the leaf surface, their importance for early events in the interaction with host-specific pathogenic fungi can now be evaluated. On adaxial surfaces, approximately 80\% of Erysiphe pisi spores germinated and 70\% differentiated appressoria. In contrast, significantly lower germination efficiencies (57\%) and appressoria formation rates (49\%) were found for abaxial surfaces. In conclusion, the influence of the physical structure and the chemical composition of the host surface, and especially of epicuticular leaf waxes, on the prepenetration processes of biotrophic fungi is discussed.},
	keywords = {APPRESSORIUM FORMATION, BLUMERIA-GRAMINIS, COLLETOTRICHUM-GLOEOSPORIOIDES, cuticle, EPICUTICULAR WAX, ERYSIPHE-GRAMINIS, GERMINATION, INTRACUTICULAR LIPIDS, LEAVES, PISUM-SATIVUM L},
	isbn = {0032-0889},
	url = {<Go to ISI>://000231765300049},
	author = {Gniwotta, F. and Vogg, G. and Gartmann, V. and Carver, T. L. W. and Riederer, M. and Jetter, R.}
}
@article {1026,
	title = {Tomato fruit cuticular waxes and their effects on transpiration barrier properties: functional characterization of a mutant deficient in a very-long-chain fatty acid beta-ketoacyl-CoA synthase},
	journal = {Journal of Experimental Botany},
	volume = {55},
	number = {401},
	year = {2004},
	note = {ISI Document Delivery No.: 829GETimes Cited: 49Cited Reference Count: 44},
	month = {Jun},
	pages = {1401-1410},
	type = {Article},
	abstract = {Cuticular waxes play a pivotal role in limiting transpirational water loss across the plant surface. The correlation between the chemical composition of the cuticular waxes and their function as a transpiration barrier is still unclear. In the present study, intact tomato fruits (Lycopersicon esculentum) are used, due to their astomatous surface, as a novel integrative approach to investigate this composition-function relationship: wax amounts and compositions of tomato were manipulated before measuring unbiased cuticular transpiration. First, successive mechanical and extractive wax-removal steps allowed the selective modification of epi- and intracuticular wax layers. The epicuticular film consisted exclusively of very-long-chain aliphatics, while the intracuticular compartment contained large quantities of pentacyclic triterpenoids as well. Second, applying reverse genetic techniques, a loss-of-function mutation with a transposon insertion in a very-long-chain fatty acid elongase beta-ketoacyl-CoA synthase was isolated and characterized. Mutant leaf and fruit waxes were deficient in n-alkanes and aldehydes with chain lengths beyond C-30, while shorter chains and branched hydrocarbons were not affected. The mutant fruit wax also showed a significant increase in intracuticular triterpenoids. Removal of the epicuticular wax layer, accounting for one-third of the total wax coverage on wild-type fruits, had only moderate effects on transpiration. By contrast, reduction of the intracuticular aliphatics in the mutant to approximately 50\% caused a 4-fold increase in permeability. Hence, the main portion of the transpiration barrier is located in the intracuticular wax layer, largely determined by the aliphatic constituents, but modified by the presence of triterpenoids, whereas epicuticular aliphatics play a minor role.},
	keywords = {ARABIDOPSIS-THALIANA, BARRIER, BICOLOR L MOENCH, CITRUS LEAF CUTICLES, CONDENSING ENZYME, CONDUCTANCE, cuticle, ECERIFERUM CER MUTANTS, EPICUTICULAR WAX, EPICUTICULAR WAXES, EPIDERMAL, intracuticular wax, PRUNUS-LAUROCERASUS L, tomato, transpiration, TRANSPORT-PROPERTIES, WATER PERMEABILITY},
	isbn = {0022-0957},
	url = {<Go to ISI>://000222034600015},
	author = {Vogg, G. and Fischer, S. and Leide, J. and Emmanuel, E. and Jetter, R. and Levy, A. A. and Riederer, M.}
}
