@article {TPJ:TPJ13294, title = {The composition of surface wax on trichomes of Arabidopsis thaliana differs from wax on other epidermal cells}, journal = {The Plant Journal}, year = {2016}, pages = {n/a{\textendash}n/a}, keywords = {Arabidopsis thaliana, cuticle, Epidermis, HYDROCARBONS, Pavement cells, trichomes, wax}, issn = {1365-313X}, doi = {10.1111/tpj.13294}, url = {http://dx.doi.org/10.1111/tpj.13294}, author = {Hegebarth, Daniela and Buschhaus, Christopher and Wu, May and Bird, David and Jetter, Reinhard} } @article {2345, title = {Phylogenetic ecology of leaf surface traits in the milkweeds (Asclepias spp.): chemistry, ecophysiology, and insect behavior}, journal = {New Phytologist}, volume = {183}, number = {3}, year = {2009}, note = {ISI Document Delivery No.: 472AKTimes Cited: 4Cited Reference Count: 89Agrawal, Anurag A. Fishbein, Mark Jetter, Reinhard Salminen, Juha-Pekka Goldstein, Jessica B. Freitag, Amy E. Sparks, Jed P.}, pages = {848-867}, type = {Article}, abstract = {P>The leaf surface is the contact point between plants and the environment and plays a crucial role in mediating biotic and abiotic interactions. Here, we took a phylogenetic approach to investigate the function, trade-offs, and evolution of leaf surface traits in the milkweeds (Asclepias). Across 47 species, we found trichome densities of up to 3000 trichomes cm(-2) and epicuticular wax crystals (glaucousness) on 10 species. Glaucous species had a characteristic wax composition dominated by very-long-chain aldehydes. The ancestor of the milkweeds was probably a glaucous species, from which there have been several independent origins of glabrous and pubescent types. Trichomes and wax crystals showed negatively correlated evolution, with both surface types showing an affinity for arid habitats. Pubescent and glaucous milkweeds had a higher maximum photosynthetic rate and lower stomatal density than glabrous species. Pubescent and glaucous leaf surfaces impeded settling behavior of monarch caterpillars and aphids compared with glabrous species, although surface types did not show consistent differentiation in secondary chemistry. We hypothesize that pubescence and glaucousness have evolved as alternative mechanisms with similar functions. The glaucous type, however, appears to be ancestral, lost repeatedly, and never regained; we propose that trichomes are a more evolutionarily titratable strategy. New Phytologist (2009) 183: 848-867doi: 10.1111/j.1469-8137.2009.02897.x.}, keywords = {adaptation to arid environments, ALDEHYDES, CARBON ISOTOPE DISCRIMINATION, cardenolides, CHARACTERS, CHEMICAL-COMPOSITION, cuticular, cuticular waxes, DISCRETE, EPICUTICULAR WAX CRYSTALS, EVOLUTION, IN-VITRO RECONSTITUTION, LEAVES, monarch butterfly caterpillar (Danaus plexippus), plant defense, STRATEGIES, syndromes, trichomes, UV PHOTOPROTECTION, water use efficiency, waxes}, isbn = {0028-646X}, url = {://000268100800031}, author = {Agrawal, A. A. and Fishbein, M. and Jetter, R. and Salminen, J. P. and Goldstein, J. B. and Freitag, A. E. and Sparks, J. P.} } @article {1126, title = {Surface composition of myrmecophilic plants: Cuticular wax and glandular trichomes on leaves of Macaranga tanarius}, journal = {Journal of Chemical Ecology}, volume = {31}, number = {10}, year = {2005}, note = {ISI Document Delivery No.: 969XKTimes Cited: 3Cited Reference Count: 30}, month = {Oct}, pages = {2323-2341}, type = {Article}, abstract = {Primary plant surfaces, covered with cuticles consisting of cutin and waxes, are important substrates for interaction with insects. The composition of leaf surfaces of the myrmecophilic plant Macaranga tanarius was studied. The prenylated flavanone nymphaeol-C was identified in surface extracts and was localized exclusively in glandular trichomes on the abaxial leaf side. The epidermal pavement cells surrounding these trichomes were covered with a smooth film of epicuticular wax from which few small wax crystals protruded. The epicuticular wax amounted to approximately 8 mu g cm(-2), corresponding to 85\% of the wax load on the adaxial as well as the abaxial leaf sides. The epicuticular wax mixtures from both leaf surfaces contained more than 70\% primary alcohols, 14\% fatty acids, 2\% aldehydes, and traces of alkyl acetates, with chain lengths ranging from C-20 to C-38. In contrast, the intracuticular wax layer was largely dominated by triterpenoid alcohols alpha-amyrin, beta-amyrin, and lupeol. Consequently, these characteristic compounds are not available for direct contact with insects on the plant surface.}, keywords = {ACID, ANT-PLANTS, BLOOMS, CHEMICAL-COMPOSITION, CREMATOGASTER, DIVERSITY, EPICUTICULAR WAX, EUPHORBIACEAE, flavanones, glandular, isoprenoids, leaf surface, Macaranga tanarius, nymphaeol-C, plant insect interactions, PRENYLFLAVANONES, trichomes}, isbn = {0098-0331}, url = {://000232269000006}, author = {Guhling, O. and Kinzler, C. and Dreyer, M. and Bringmann, G. and Jetter, R.} }