@article {2438,
	title = {A DNAzyme with Three Protein-Like Functional Groups: Enhancing Catalytic Efficiency of M2+-Independent RNA Cleavage},
	journal = {Chembiochem},
	volume = {10},
	number = {12},
	year = {2009},
	note = {ISI Document Delivery No.: 487MWTimes Cited: 5Cited Reference Count: 76Hollenstein, Marcel Hipolito, Christopher J. Lam, Curtis H. Perrin, David M.},
	month = {Aug},
	pages = {1988-1992},
	type = {Article},
	keywords = {BEACON SENSOR, biology, CLEAVING DNA ENZYME, CYTOSOLIC FREE MAGNESIUM, DEOXYRIBOZYMES, DNAzymes, enzyme catalysis, EVOLUTION, HAIRPIN RIBOZYME, HIGH-DENSITY, IN-VITRO SELECTION, ION, nucleic acids, NUCLEIC-ACIDS, RNAse A mimics, synthetic},
	isbn = {1439-4227},
	url = {<Go to ISI>://000269279700012},
	author = {Hollenstein, M. and Hipolito, C. J. and Lam, C. H. and Perrin,David M.}
}
@article {2343,
	title = {Fruit-Surface Flavonoid Accumulation in Tomato Is Controlled by a SIMYB12-Regulated Transcriptional Network},
	journal = {Plos Genetics},
	volume = {5},
	number = {12},
	year = {2009},
	note = {ISI Document Delivery No.: 542CTTimes Cited: 3Cited Reference Count: 58Adato, Avital Mandel, Tali Mintz-Oron, Shira Venger, Ilya Levy, Dorit Yativ, Merav Dominguez, Eva Wang, Zhonghua De Vos, Ric C. H. Jetter, Reinhard Schreiber, Lukas Heredia, Antonio Rogachev, Ilana Aharoni, Asaph},
	month = {Dec},
	pages = {23},
	type = {Article},
	abstract = {The cuticle covering plants{\textquoteright} aerial surfaces is a unique structure that plays a key role in organ development and protection against diverse stress conditions. A detailed analysis of the tomato colorless-peel y mutant was carried out in the framework of studying the outer surface of reproductive organs. The y mutant peel lacks the yellow flavonoid pigment naringenin chalcone, which has been suggested to influence the characteristics and function of the cuticular layer. Large-scale metabolic and transcript profiling revealed broad effects on both primary and secondary metabolism, related mostly to the biosynthesis of phenylpropanoids, particularly flavonoids. These were not restricted to the fruit or to a specific stage of its development and indicated that the y mutant phenotype is due to a mutation in a regulatory gene. Indeed, expression analyses specified three R2R3-MYB-type transcription factors that were significantly down-regulated in the y mutant fruit peel. One of these, SIMYB12, was mapped to the genomic region on tomato chromosome 1 previously shown to harbor the y mutation. Identification of an additional mutant allele that co-segregates with the colorless-peel trait, specific down-regulation of SlMYB12 and rescue of the y phenotype by overexpression of SlMYB12 on the mutant background, confirmed that a lesion in this regulator underlies the y phenotype. Hence, this work provides novel insight to the study of fleshy fruit cuticular structure and paves the way for the elucidation of the regulatory network that controls flavonoid accumulation in tomato fruit cuticle.},
	keywords = {AFFECTS VEGETATIVE DEVELOPMENT, ARABIDOPSIS-THALIANA, EVOLUTION, EXPRESSION, GENE, IDENTIFICATION, LIQUID-CHROMATOGRAPHY, METABOLISM, PHENYLPROPANOID BIOSYNTHESIS, REGULATOR},
	isbn = {1553-7390},
	url = {<Go to ISI>://000273469700027},
	author = {Adato, A. and Mandel, T. and Mintz-Oron, S. and Venger, I. and Levy, D. and Yativ, M. and Dominguez, E. and Wang, Z. H. and De Vos, R. C. H. and Jetter, R. and Schreiber, L. and Heredia, A. and Rogachev, I. and Aharoni, A.}
}
@article {2345,
	title = {Phylogenetic ecology of leaf surface traits in the milkweeds (Asclepias spp.): chemistry, ecophysiology, and insect behavior},
	journal = {New Phytologist},
	volume = {183},
	number = {3},
	year = {2009},
	note = {ISI Document Delivery No.: 472AKTimes Cited: 4Cited Reference Count: 89Agrawal, Anurag A. Fishbein, Mark Jetter, Reinhard Salminen, Juha-Pekka Goldstein, Jessica B. Freitag, Amy E. Sparks, Jed P.},
	pages = {848-867},
	type = {Article},
	abstract = {P>The leaf surface is the contact point between plants and the environment and plays a crucial role in mediating biotic and abiotic interactions. Here, we took a phylogenetic approach to investigate the function, trade-offs, and evolution of leaf surface traits in the milkweeds (Asclepias). Across 47 species, we found trichome densities of up to 3000 trichomes cm(-2) and epicuticular wax crystals (glaucousness) on 10 species. Glaucous species had a characteristic wax composition dominated by very-long-chain aldehydes. The ancestor of the milkweeds was probably a glaucous species, from which there have been several independent origins of glabrous and pubescent types. Trichomes and wax crystals showed negatively correlated evolution, with both surface types showing an affinity for arid habitats. Pubescent and glaucous milkweeds had a higher maximum photosynthetic rate and lower stomatal density than glabrous species. Pubescent and glaucous leaf surfaces impeded settling behavior of monarch caterpillars and aphids compared with glabrous species, although surface types did not show consistent differentiation in secondary chemistry. We hypothesize that pubescence and glaucousness have evolved as alternative mechanisms with similar functions. The glaucous type, however, appears to be ancestral, lost repeatedly, and never regained; we propose that trichomes are a more evolutionarily titratable strategy. New Phytologist (2009) 183: 848-867doi: 10.1111/j.1469-8137.2009.02897.x.},
	keywords = {adaptation to arid environments, ALDEHYDES, CARBON ISOTOPE DISCRIMINATION, cardenolides, CHARACTERS, CHEMICAL-COMPOSITION, cuticular, cuticular waxes, DISCRETE, EPICUTICULAR WAX CRYSTALS, EVOLUTION, IN-VITRO RECONSTITUTION, LEAVES, monarch butterfly caterpillar (Danaus plexippus), plant defense, STRATEGIES, syndromes, trichomes, UV PHOTOPROTECTION, water use efficiency, waxes},
	isbn = {0028-646X},
	url = {<Go to ISI>://000268100800031},
	author = {Agrawal, A. A. and Fishbein, M. and Jetter, R. and Salminen, J. P. and Goldstein, J. B. and Freitag, A. E. and Sparks, J. P.}
}
@article {2017,
	title = {Recombination of protein fragments: A promising approach toward engineering proteins with novel nanomechanical properties},
	journal = {Protein Science},
	volume = {17},
	number = {10},
	year = {2008},
	note = {ISI Document Delivery No.: 351CKTimes Cited: 4Cited Reference Count: 50Balamurali, M. M. Sharma, Deepak Chang, Anderson Khor, Dingyue Chu, Ricky Li, Hongbin},
	month = {Oct},
	pages = {1815-1826},
	type = {Article},
	abstract = {Combining single molecule atomic force microscopy (AFM) and protein engineering techniques, here we demonstrate that we can use recombination-based techniques to engineer novel elastomeric proteins by recombining protein fragments from structurally homologous parent proteins. Using I27 and I32 domains from the muscle protein titin as parent template proteins, we systematically shuffled the secondary structural elements of the two parent proteins and engineered 13 hybrid daughter proteins. Although I27 and I32 are highly homologous, and homology modeling predicted that the hybrid daughter proteins fold into structures that are similar to that of parent protein, we found that only eight of the 13 daughter proteins showed beta-sheet dominated structures that are similar to parent proteins, and the other five recombined proteins showed signatures of the formation of significant alpha-helical or random coil-like structure. Single molecule AFM revealed that six recombined daughter proteins are mechanically stable and exhibit mechanical properties that are different from the parent proteins. In contrast, another four of the hybrid proteins were found to be mechanically labile and unfold at forces that are lower than the similar to 20 pN, as we could not detect any unfolding force peaks. The last three hybrid proteins showed interesting duality in their mechanical unfolding behaviors. These results demonstrate the great potential of using recombination-based approaches to engineer novel elastomeric protein domains of diverse mechanical properties. Moreover, our results also revealed the challenges and complexity of developing a recombination-based approach into a laboratory-based directed evolution approach to engineer novel elastomeric proteins.},
	keywords = {ATOMIC-FORCE MICROSCOPY, BIOLOGICAL ROLES, COMPUTATIONAL DESIGN, ELASTICITY, elastomeric protein, EVOLUTION, IMMUNOGLOBULIN DOMAINS, MECHANICAL STABILITY, mechanical unfolding, MOLECULAR-DYNAMICS SIMULATION, recombination, SEQUENCE, SINGLE PROTEIN, single-molecule force spectroscopy, TITIN},
	isbn = {0961-8368},
	url = {<Go to ISI>://000259401900019},
	author = {Balamurali, M. M. and Sharma, D. and Chang, A. and Khor, D. and Chu, R. and Li, H. B.}
}
@article {1555,
	title = {Engineering proteins with novel mechanical properties by recombination of protein fragments},
	journal = {Angewandte Chemie-International Edition},
	volume = {45},
	number = {34},
	year = {2006},
	note = {ISI Document Delivery No.: 082MRTimes Cited: 20Cited Reference Count: 51Sharma, Deepak Cao, Yi Li, Hongbin},
	pages = {5633-5638},
	type = {Article},
	keywords = {AFM, ATOMIC-FORCE MICROSCOPY, ELASTICITY, EVOLUTION, IG DOMAIN, IMMUNOGLOBULIN DOMAINS, MOLECULAR-DYNAMICS SIMULATIONS, protein engineering, protein structures, scanning probe microscopy, SINGLE PROTEIN, single-molecule studies, SPECTROSCOPY, STABILITY, TITIN},
	isbn = {1433-7851},
	url = {<Go to ISI>://000240391400016},
	author = {Sharma, D. and Cao, Y. and Li, H. B.}
}
@article {5138,
	title = {The structure of L-ribulose-5-phosphate 4-epimerase: An aldolase-like platform for epimerization},
	journal = {Biochemistry},
	volume = {40},
	number = {49},
	year = {2001},
	note = {ISI Document Delivery No.: 500CJTimes Cited: 18Cited Reference Count: 45},
	month = {Dec},
	pages = {14763-14771},
	type = {Article},
	abstract = {The structure of L-ribulose-5 -phosphate 4-epimerase from E. coli has been solved to 2.4 Angstrom resolution using X-ray diffraction data. The structure is homo-tetrameric and displays C-4 symmetry. Each subunit has a single domain comprised of a central beta -sheet flanked on either side by layers of alpha -helices. The active site is identified by the position of the catalytic zinc residue and is located at the interface between two adjacent subunits. A remarkable feature of the structure is that it shows a very close resemblance to that of L-fuculose-1-phosphate aldolase. This is consistent with the notion that both enzymes belong to a superfamily of epimerases/aldolases that catalyze carbon-carbon bond cleavage reactions via a metal-stabilized enolate intermediate. Detailed inspection of the epimerase structure, however, indicates that despite the close overall structural similarity to class II aldolases, the enzyme has evolved distinct active site features that promote its particular chemistry.},
	keywords = {CHEMISTRY, CLASS-II, CLEAVAGE, ENZYME, ESCHERICHIA-COLI, EVOLUTION, GALACTOSE, L-FUCULOSE-1-PHOSPHATE ALDOLASE, MECHANISM, SEQUENCE, SUPERFAMILIES},
	isbn = {0006-2960},
	url = {<Go to ISI>://000172608100005},
	author = {Luo, Y. and Samuel, J. and Mosimann, S. C. and Lee, J. E. and Tanner, M. E. and Strynadka, N. C. J.}
}
@article {3772,
	title = {Survival probability for the Yamaguchi potential},
	journal = {Annals of Physics},
	volume = {252},
	number = {2},
	year = {1996},
	note = {ISI Document Delivery No.: VY568Times Cited: 14Cited Reference Count: 40},
	month = {Dec},
	pages = {336-356},
	type = {Article},
	abstract = {Different decay behaviours of the survival probability are examined. Technically, the survival probability of an arbitrary state psi is determined by the psi matrix element of the resolvent of the full Hamiltonian. The analytical properties of this matrix element can be analyzed in terms of the properties of the kernel of the Lippmann-Schwinger equation. For the Yamaguchi potential and several initial states, all functions are explicitly calculated. This approach allows the decomposition of the survival amplitude into a sum of decaying exponential terms and w-functions associated with the pole positions of the resolvent matrix element in the complex momentum plane. Novel decay behaviours are found for the decay of states associated with the resolvent poles and the decay of a state which is dominated by the form of the wave function rather than by the resolvent poles. Certain anomalous short time decay behaviour is also exemplified. (C) 1996 Academic Press, Inc.},
	keywords = {EVOLUTION, EXPONENTIAL DECAY LAW, MOMENTUM REPRESENTATION, QUANTUM, resonant states, time},
	isbn = {0003-4916},
	url = {<Go to ISI>://A1996VY56800005},
	author = {Muga, J. G. and Wei, G. W. and Snider, R. F.}
}
