@article {2645,
	title = {Composition of the epicuticular and intracuticular wax layers on Kalanchoe daigremontiana (Hamet et Perr. de la Bathie) leaves},
	journal = {Phytochemistry},
	volume = {70},
	number = {7},
	year = {2009},
	note = {ISI Document Delivery No.: 475UKTimes Cited: 1Cited Reference Count: 26van Maarseveen, Clare Jetter, Reinhard},
	month = {May},
	pages = {899-906},
	type = {Article},
	abstract = {Epicuticular and intracuticular waxes from both adaxial and abaxial surfaces of the leaves of Kalanchoe daigremontiana were analyzed. All wax mixtures were found to contain approximately equal amounts of triterpenoids and very long chain fatty acid (VLCFA) derivatives. The triterpenoid fraction consisted of glutinol (8-19\% of the total wax) and friedelin (4-9\%), together with smaller amounts of glutanol, glutinol acetate, epifriedelanol, germanicol and beta-amyrin. The VLCFA derivatives comprised C-27-C-35 alkanes (19-37\% of the total wax), C-32-C-34 aldehydes (3-7\%), C-32 and C-34 fatty acids (0.2-3\%), C-26-C-36 primary alcohols (4-8\%), and C-42-C-52 alkyl esters (2-9\%). The wax layers were found to differ in triterpenoid amounts, with the intracuticular wax containing higher percentages of most triterpenoids than the epicuticular wax. Friedelin, the only triterpenoid ketone present, showed the opposite distribution with higher proportions in the epicuticular wax. VLCFA derivatives also accumulated to higher percentages in the epicuticular than in the intracuticular wax layer. Epicuticular wax crystals were observed on both the adaxial and abaxial leaf surfaces. (C) 2009 Elsevier Ltd. All rights reserved.},
	keywords = {chain length, CHEMICAL-COMPOSITION, Crassulaceae, DUDLEYA, friedelin, glutinol, Kalanchoe daigremontiana, LEAF CUTICULAR WAXES, leaf surface, PINNATA, PLANTS, STEROLS, SURFACES, SYNTHASE, triterpenoids, Very long chain fatty acids},
	isbn = {0031-9422},
	url = {<Go to ISI>://000268386700009},
	author = {van Maarseveen, C. and Jetter, R.}
}
@article {1626,
	title = {Nanotubules on plant surfaces: Chemical composition of epicuticular wax crystals on needles of Taxus baccata L},
	journal = {Phytochemistry},
	volume = {67},
	number = {16},
	year = {2006},
	note = {ISI Document Delivery No.: 090QVTimes Cited: 13Cited Reference Count: 24Wen, Miao Buschhaus, Christopher Jetter, Reinhard},
	month = {Aug},
	pages = {1808-1817},
	type = {Article},
	abstract = {Needles of Taxus baccata L. were covered with tubular epicuticular wax crystals varying in diameters (100 and 250 nm) and lengths (300-500 and 500-1000 nm) on the abaxial and adaxial surfaces, respectively. Various sampling protocols were employed to study the chemical composition of the needle waxes on three different levels of spatial resolution. First, a dipping extraction of whole needles yielded the total cuticular wax mixture consisting of very long chain fatty acids (21\%), alkanediols (19\%), phenyl esters (15\%), and secondary alcohols (9\%) together with small amounts of aldehydes, primary alcohols, alkanes, alkyl esters, and tocopherols. Second, waxes from both sides of the needle were sampled separately by brushing with CHCl3-soaked fabric glass. Both sides showed very similar qualitative composition, but differed drastically in quantitative aspects, with nonacosan-10-ol (18\%) and alkanediols (33\%) dominating the abaxial and adaxial waxes, respectively. Third, the epi- and intracuticular wax layers were selectively sampled by a combination of mechanical wax removal and brushing extraction. This provided direct evidence that the tubular wax crystals contained high percentages of nonacosane-4,10-diol and nonacosane-5,10-diol on the abaxial surface, and nonacosan-10-ol on the adaxial surface of the needles. Together with these compounds, relatively large amounts of fatty acids and smaller percentages of aldehydes, primary alcohols, alkyl esters, and alkanes co-crystallized in the epicuticular layer. In comparison, the intracuticular wax consisted of higher portions of cyclic constituents and aliphatics with relatively high polarity. The formation of the tubular crystals is discussed as a spontaneous physicochemical process, involving the establishment of gradients between the epi- and intracuticular wax layers and local phase separation. (c) 2006 Elsevier Ltd. All rights reserved.},
	keywords = {composition, CUTICULAR WAX, IN-VITRO RECONSTITUTION, LEAF CUTICULAR WAXES, LEAVES, SECONDARY ALCOHOLS, SURFACE, Taxaceae, Taxus baccata, yew needles},
	isbn = {0031-9422},
	url = {<Go to ISI>://000240966900015},
	author = {Wen, M. and Buschhaus, C. and Jetter, R.}
}
