@article {998,
	title = {Identification and quantification of arsC genes in environmental samples by using real-time PCR},
	journal = {Journal of Microbiological Methods},
	volume = {58},
	number = {3},
	year = {2004},
	note = {ISI Document Delivery No.: 847SSTimes Cited: 24Cited Reference Count: 34},
	month = {Sep},
	pages = {335-349},
	type = {Article},
	abstract = {The arsC gene is responsible for the first step in arsenate biotransformation encoding the enzyme arsenate reductase. The quantitative real-time PCR method was developed to quantify the abundance of the arsC genes in environmental samples contaminated with arsenic. Two sets of primers that showed high specificity for the target arsC gene were designed based on consensus sequences from 13 bacterial species. The arsC gene was used as an external standard instead of total DNA in the calibration curve for real-time PCR, which was linear over six orders of magnitude and the detection limit was estimated to be about three copies of the gene. Soil samples from arsenic contaminated sites were screened for arsC genes by using PCR and showed the presence of this gene. The copy numbers of the gene ranging from 0.88 x 10(4) to 1.56 x 10(5) per ng total DNA were found in eight arsenic contaminated samples. Soil samples from a bioreactor containing pulp mill biomass and high concentration of arsenate showed a tenfold higher count of arsC gene copies than soil samples collected underground from an arsenic-rich gold mine. (C) 2004 Elsevier B.V. All rights reserved.},
	keywords = {arsC gene, arsenate reduction, ASSAY, BACTERIA, DNA, ESCHERICHIA-COLI, group-specific primers, QUANTITATION, real-time PCR, REDUCTION, resistance, SOIL, SYBR (R) Green 1},
	isbn = {0167-7012},
	url = {<Go to ISI>://000223415800006},
	author = {Sun, Y. M. and Polishchuk, E. A. and Radoja, U. and Cullen, W. R.}
}
@article {966,
	title = {The mechanism of the reaction catalyzed by ADP-beta-L-glycero-D-manno-heptose 6-epimerase},
	journal = {Journal of the American Chemical Society},
	volume = {126},
	number = {29},
	year = {2004},
	note = {ISI Document Delivery No.: 840JTTimes Cited: 14Cited Reference Count: 24},
	month = {Jul},
	pages = {8878-8879},
	type = {Article},
	keywords = {4, 6-DEHYDRATASE, BACTERIA, D-MANNOHEPTOSE 6-EPIMERASE, EPIMERIZATION, ESCHERICHIA-COLI, GLUCOSE, INSIGHTS, NUCLEOTIDE-MODIFYING ENZYMES, OUTER-MEMBRANE, UDP-GALACTOSE 4-EPIMERASE},
	isbn = {0002-7863},
	url = {<Go to ISI>://000222855300009},
	author = {Read, J. A. and Ahmed, R. A. and Morrison, J. P. and Coleman, W. G. and Tanner, M. E.}
}
@article {335,
	title = {The power distribution advantage of fiber-optic coupled ultraviolet resonance Raman spectroscopy for bioanalytical and biomedical applications},
	journal = {Journal of Raman Spectroscopy},
	volume = {33},
	number = {7},
	year = {2002},
	note = {ISI Document Delivery No.: 580KBTimes Cited: 18Cited Reference Count: 51},
	month = {Jul},
	pages = {503-510},
	type = {Article},
	abstract = {Fiber-optic coupled ultraviolet resonance Raman spectroscopy (FO-UVRRS) of photosensitive biological samples is discussed in the context of both clinical and basic medical research applications. The fiberoptic probes are designed specifically for resonance Raman spectroscopy and offer a power distribution advantage over conventional focusing UVRR spectrometers that allows the use of higher power levels without increasing the risk of photo-damage. A typical fiber-optic probe using a 600 pm core diameter fiber for illumination of the sample allows an increase in power at the sample of over an order of magnitude while maintaining the same power density, and therefore the same level of safety, as a typical conventional UVRR spectrometer. This allows high-quality spectra to be easily obtained in 10 s. Spectra of representative biological samples using high power levels without damage are presented, including the study of a photosensitive enzyme-substrate system under anaerobic conditions. Copyright (C) 2002 John Wiley Sons, Ltd.},
	keywords = {ACTIVATION, AROMATIC-AMINO-ACIDS, BACTERIA, DEEP UV, ENZYMES, LASER EXCITATION, PROBE, SIMPLICITY, SPECTRA, TISSUE},
	isbn = {0377-0486},
	url = {<Go to ISI>://000177232800003},
	author = {Barbosa, C. J. and Vaillancourt, F. H. and Eltis, L. D. and Blades, M. W. and Turner, R. F. B.}
}
@article {335,
	title = {The power distribution advantage of fiber-optic coupled ultraviolet resonance Raman spectroscopy for bioanalytical and biomedical applications},
	journal = {Journal of Raman Spectroscopy},
	volume = {33},
	number = {7},
	year = {2002},
	note = {ISI Document Delivery No.: 580KBTimes Cited: 18Cited Reference Count: 51},
	month = {Jul},
	pages = {503-510},
	type = {Article},
	abstract = {Fiber-optic coupled ultraviolet resonance Raman spectroscopy (FO-UVRRS) of photosensitive biological samples is discussed in the context of both clinical and basic medical research applications. The fiberoptic probes are designed specifically for resonance Raman spectroscopy and offer a power distribution advantage over conventional focusing UVRR spectrometers that allows the use of higher power levels without increasing the risk of photo-damage. A typical fiber-optic probe using a 600 pm core diameter fiber for illumination of the sample allows an increase in power at the sample of over an order of magnitude while maintaining the same power density, and therefore the same level of safety, as a typical conventional UVRR spectrometer. This allows high-quality spectra to be easily obtained in 10 s. Spectra of representative biological samples using high power levels without damage are presented, including the study of a photosensitive enzyme-substrate system under anaerobic conditions. Copyright (C) 2002 John Wiley Sons, Ltd.},
	keywords = {ACTIVATION, AROMATIC-AMINO-ACIDS, BACTERIA, DEEP UV, ENZYMES, LASER EXCITATION, PROBE, SIMPLICITY, SPECTRA, TISSUE},
	isbn = {0377-0486},
	url = {<Go to ISI>://000177232800003},
	author = {Barbosa, C. J. and Vaillancourt, F. H. and Eltis, L. D. and Blades, M. W. and Turner, R. F. B.}
}
@article {441,
	title = {Sequential anaerobic-aerobic treatment of soil contaminated with weathered aroclor 1260},
	journal = {Environmental Science \& Technology},
	volume = {36},
	number = {1},
	year = {2002},
	note = {ISI Document Delivery No.: 509RCTimes Cited: 25Cited Reference Count: 36},
	month = {Jan},
	pages = {100-103},
	type = {Article},
	abstract = {Soil contaminated with weathered Aroclor 1260 was bioremediated by sequential anaerobic and aerobic laboratory-scale treatment The initial concentration was 59 mug of PCBs/g of soil. Following 4 months of anaerobic treatment with an enrichment culture, all of the major components in Aroclor 1260 were completely or partially transformed to less chlorinated PCB congeners. The major products of reductive dechlorination were 24-24-tetrachlorobiphenyl and 24-26-tetrachlorobiphenyl, and the average chlorine substituents per PCB molecule decreased from 6.4 to 5.2. The molar concentration of PCBs did not decrease during the anaerobic treatment. All of the major products formed during the anaerobic treatment were degraded in the subsequent aerobic treatment using Burkholderia sp. strain LB400. After 28 days of the aerobic treatment, the concentration of PCBs was reduced to 20 mug/g of soil. PCBs were not significantly removed in aerobic treatments unless they were bioaugmented with LB400. Also, PCB degradation was not detected in soil bioaugmented with LB400 without prior anaerobic treatment. These results confirm the potential for extensive biological destruction of highly chlorinated, weathered PCB congeners in soil.},
	keywords = {BACTERIA, BIODEGRADATION, CLONING, ENCODING BIPHENYL, EXPRESSION, GENE-CLUSTER, PCBS, POLYCHLORINATED BIPHENYL-DEGRADATION, PSEUDOMONAS STRAIN LB400, REDUCTIVE DECHLORINATION},
	isbn = {0013-936X},
	url = {<Go to ISI>://000173162600031},
	author = {Master, E. R. and Lai, V. W. M. and Kuipers, B. and Cullen, W. R. and Mohn, W. W.}
}
@article {5071,
	title = {Evidence for the biosynthesis of squalene via the methylerythritol phosphate pathway in a Streptomyces sp obtained from a marine sediment},
	journal = {Journal of Organic Chemistry},
	volume = {66},
	number = {18},
	year = {2001},
	note = {ISI Document Delivery No.: 470LVTimes Cited: 6Cited Reference Count: 23},
	month = {Sep},
	pages = {6202-6206},
	type = {Article},
	keywords = {2-C-METHYL-D-ERYTHRITOL, 4-(CYTIDINE, 4-PHOSPHATE, 5{\textquoteright}-DIPHOSPHO)-2-C-METHYL-D-ERYTHRITOL, BACTERIA, HIGHER-PLANTS, ISOPRENOID BIOSYNTHESIS, MEVALONATE-INDEPENDENT PATHWAY, NONMEVALONATE PATHWAY, PRECURSOR, terpenoids},
	isbn = {0022-3263},
	url = {<Go to ISI>://000170874200039},
	author = {Fontana, A. and Kelly, M. T. and Prasad, J. D. and Andersen, R. J.}
}
@article {3982,
	title = {Massetolides A-H, antimycobacterial cyclic depsipeptides produced by two pseudomonads isolated from marine habitats},
	journal = {Journal of Natural Products},
	volume = {60},
	number = {3},
	year = {1997},
	note = {ISI Document Delivery No.: WP603Times Cited: 52Cited Reference Count: 16},
	month = {Mar},
	pages = {223-229},
	type = {Article},
	abstract = {Massetolides A-H (1-8), novel cyclic depsipetides, and the known compound viscosin (9) have been isolated from cultures of two Pseudomonas sp. isolated from a marine alga and a marine tube worm, respectively. Massetolide A (1) and viscosin (9) exhibit in vitro antimicrobial activity against Mycobacterium tuberculosis and Mycobacterium avium-intracellulare. Precursor-directed biosynthesis has been used to generate unnatural massetolides 11-13 incorporating nonprotein amino acids.},
	keywords = {BACTERIA, biosynthesis, METABOLITES},
	isbn = {0163-3864},
	url = {<Go to ISI>://A1997WP60300006},
	author = {Gerard, J. and Lloyd, R. and Barsby, T. and Haden, P. and Kelly, M. T. and Andersen, R. J.}
}
@article {3510,
	title = {ARTIFICIAL NEURAL-NETWORK AND CLASSICAL LEAST-SQUARES METHODS FOR NEUROTRANSMITTER MIXTURE ANALYSIS},
	journal = {Journal of Neuroscience Methods},
	volume = {56},
	number = {2},
	year = {1995},
	note = {ISI Document Delivery No.: QH949Times Cited: 5Cited Reference Count: 28},
	month = {Feb},
	pages = {155-167},
	type = {Article},
	abstract = {Identification of individual components in biological mixtures can be a difficult problem regardless of the analytical method employed. In this work, Raman spectroscopy was chosen as a prototype analytical method due to its inherent versatility and applicability to aqueous media, making it useful for the study of biological samples. Artificial neural networks (ANNs) and the classical least-squares (CLS) method were used to identify and quantify the Raman spectra of the small-molecule neurotransmitters and mixtures of such molecules. The transfer functions used by a network, as well as the architecture of a network, played an important role in the ability of the network to identify the Raman spectra of individual neurotransmitters and the Raman spectra of neurotransmitter mixtures. Specifically, networks using sigmoid and hyperbolic tangent transfer functions generalized better from the mixtures in the training data set to those in the testing data sets than networks using sine functions. Networks with connections that permit the local processing of inputs generally performed better than other networks on all the testing data sets, and better than the CLS method of curve fitting, on novel spectra of some neurotransmitters. The CLS method was found to perform well on noisy, shifted, and difference spectra.},
	keywords = {ARTIFICIAL NEURAL, BACTERIA, CLASSICAL LEAST-SQUARES ANALYSIS, INVIVO VOLTAMMETRY, MIXTURE ANALYSIS, NETWORK, Raman spectroscopy, REGRESSION, SMALL-MOLECULE NEUROTRANSMITTER, SPECTRA, SYSTEM, UV RESONANCE RAMAN},
	isbn = {0165-0270},
	url = {<Go to ISI>://A1995QH94900006},
	author = {Schulze, H. G. and Greek, L. S. and Gorzalka, B. B. and Bree, A. V. and Blades, M. W. and Turner, R. F. B.}
}
@article {3510,
	title = {ARTIFICIAL NEURAL-NETWORK AND CLASSICAL LEAST-SQUARES METHODS FOR NEUROTRANSMITTER MIXTURE ANALYSIS},
	journal = {Journal of Neuroscience Methods},
	volume = {56},
	number = {2},
	year = {1995},
	note = {ISI Document Delivery No.: QH949Times Cited: 5Cited Reference Count: 28},
	month = {Feb},
	pages = {155-167},
	type = {Article},
	abstract = {Identification of individual components in biological mixtures can be a difficult problem regardless of the analytical method employed. In this work, Raman spectroscopy was chosen as a prototype analytical method due to its inherent versatility and applicability to aqueous media, making it useful for the study of biological samples. Artificial neural networks (ANNs) and the classical least-squares (CLS) method were used to identify and quantify the Raman spectra of the small-molecule neurotransmitters and mixtures of such molecules. The transfer functions used by a network, as well as the architecture of a network, played an important role in the ability of the network to identify the Raman spectra of individual neurotransmitters and the Raman spectra of neurotransmitter mixtures. Specifically, networks using sigmoid and hyperbolic tangent transfer functions generalized better from the mixtures in the training data set to those in the testing data sets than networks using sine functions. Networks with connections that permit the local processing of inputs generally performed better than other networks on all the testing data sets, and better than the CLS method of curve fitting, on novel spectra of some neurotransmitters. The CLS method was found to perform well on noisy, shifted, and difference spectra.},
	keywords = {ARTIFICIAL NEURAL, BACTERIA, CLASSICAL LEAST-SQUARES ANALYSIS, INVIVO VOLTAMMETRY, MIXTURE ANALYSIS, NETWORK, Raman spectroscopy, REGRESSION, SMALL-MOLECULE NEUROTRANSMITTER, SPECTRA, SYSTEM, UV RESONANCE RAMAN},
	isbn = {0165-0270},
	url = {<Go to ISI>://A1995QH94900006},
	author = {Schulze, H. G. and Greek, L. S. and Gorzalka, B. B. and Bree, A. V. and Blades, M. W. and Turner, R. F. B.}
}
@article {3106,
	title = {CLONING, SEQUENCING, AND VISCOMETRIC ADHESION ANALYSIS OF HEAT-RESISTANT AGGLUTININ 1, AN INTEGRAL MEMBRANE HEMAGGLUTININ FROM ESCHERICHIA-COLI-O9-H10-K99},
	journal = {Infection and Immunity},
	volume = {62},
	number = {11},
	year = {1994},
	note = {ISI Document Delivery No.: PN304Times Cited: 13Cited Reference Count: 45},
	month = {Nov},
	pages = {5020-5026},
	type = {Article},
	abstract = {The gene encoding a mannose-resistant hemagglutinating protein was cloned from Escherichia coli 09:H10:K99. The hemagglutinin is different from two other mannose-resistant hemagglutinins in this strain, K99 and F41. The agglutinin, named heat-resistant agglutinin 1 (HRA1) since heating to 70 degrees C does not destroy its aggregative properties, strongly agglutinates human, pig, and dog erythrocytes, shows little or no affinity towards cow and chicken erythrocytes, but agglutinates human colon adenocarcinoma 201 (COLO 201) cells. The hra1 gene present on the recombinant plasmid pETE1 was localized by subcloning, and its nucleotide sequence was determined. The gene consists of a 792-bp open reading frame coding for a putative protein of 29 kDa with a predicted N-terminal secretory signal sequence. HRA1 shares no significant identity with data base protein sequences. HRA1 is strongly associated with the bacterial membrane, resisting sonication and isolation attempts based upon standard adhesin purification techniques. N-terminal sequencing of a unique 25-kDa band present in polyacrylamide gels of outer membrane preparations of bacteria harboring pETE1 correlated with the predicted N-terminal amino acid sequence of HRA1 after cleavage of the signal peptide. A viscometric agglutination assay sensitive to the strength of bacterial adhesion shows that the agglutination mediated by bacteria expressing HRA1 is weaker than that of bacteria bearing the F41 adhesin, probably because of the high-molecular-weight, multivalent nature of the latter adhesin. Our observations suggest that HRA1 is a monomeric outer membrane agglutinin.},
	keywords = {ANTIGEN, BACTERIA, DNA, K99, LOCALIZATION, NONFIMBRIAL, OUTER-MEMBRANE, PROTEINS, PURIFICATION, STRAINS},
	isbn = {0019-9567},
	url = {<Go to ISI>://A1994PN30400046},
	author = {Lutwyche, P. and Rupps, R. and Cavanagh, J. and Warren, R. A. J. and Brooks, D. E.}
}
