@article {444,
	title = {Carbohydrate-binding modules recognize fine substructures of cellulose},
	journal = {Journal of Biological Chemistry},
	volume = {277},
	number = {52},
	year = {2002},
	note = {ISI Document Delivery No.: 631QDTimes Cited: 20Cited Reference Count: 36},
	month = {Dec},
	pages = {50245-50254},
	type = {Article},
	abstract = {Competition isotherms are used to identify the set of cellulose substructures to which cellulose binding modules (CBMs) from families 2a, 3, 4, 9, and 17 bind. The experiments are based on coupling a unique fluorescent tag to each CBM in a manner that does not alter the natural binding properties of the CBM and therefore allows the surface and solution concentrations of each CBM to be monitored as a function of time and composition. Adsorption and surface exchange of like or competing CBMs are monitored using a range of cellulose preparations varying in both crystallinity and provenance. CBMs from families 2a, 3,4,9, and 17 are shown to recognize different physical forms of prepared cellulose. The demonstration of the very fine binding specificity of cellulose-specific CBMs implies that the polysaccharide targets of CBMs extend down to the resolution of cellulose microstructures.},
	keywords = {BACTERIAL, CELLULASE, CRYSTALLINE CELLULOSE, DOMAIN, EXOGLUCANASE, FIMI CENC, NMR-SPECTROSCOPY, PROTEIN-A, SPECIFICITY, TRYPTOPHAN RESIDUES, XYLANASE 10A},
	isbn = {0021-9258},
	url = {<Go to ISI>://000180177700007},
	author = {McLean, B. W. and Boraston, A. B. and Brouwer, D. and Sanaie, N. and Fyfe, C. A. and Warren, R. A. J. and Kilburn, D. G. and Haynes, C. A.}
}
@article {3550,
	title = {ADHESION OF MAMMALIAN-CELLS TO A RECOMBINANT ATTACHMENT FACTOR, CBD/RGD, ANALYZED BY IMAGE-ANALYSIS},
	journal = {Biotechnology and Bioengineering},
	volume = {46},
	number = {3},
	year = {1995},
	note = {ISI Document Delivery No.: QR821Times Cited: 16Cited Reference Count: 26},
	month = {May},
	pages = {185-193},
	type = {Article},
	abstract = {A luminance thresholding procedure was developed to quantify cell attachment of a variety of cell lines to CBD/RGD, a hybrid attachment factor comprising a cellulose binding domain and the fibronectin-like RGD attachment peptide. The technique used local thresholding, median filtering, and opening to separate and count cells on each image. Cell lines exhibited three different patterns of attachment to CBD/RGD, depending on whether it was immobilized on polystyrene or cellulose acetate. Vero, COS, HFF, 3T3, 293, and U373 cells attached well to CBD/RGD immobilized on polystyrene or cellulose acetate. CHO, MRC-5, and HEp-2 cells attached to CBD/RGD immobilized on polystyrene, but not to CBD/RGD immobilized on cellulose acetate. BHK and L cells failed to attach to CBD/RGD immobilized on either polystyrene or cellulose acetate. The attachment of many cell lines to CBD/RGD was comparable with attachment of these cells to fibronectin. (C) 1995 John Wiley \& Sons, Inc.},
	keywords = {ALPHA-V, ARG-GLY-ASP, CELL ATTACHMENT, CELLULASE, CELLULOMONAS FIMI, FIBRONECTIN, FRAGMENT, GROWTH, HEPARIN-BINDING, IMAGE ANALYSIS, MAMMALIAN CELLS, RECEPTOR, RECOGNITION SIGNAL, SURFACES, VITRONECTIN},
	isbn = {0006-3592},
	url = {<Go to ISI>://A1995QR82100001},
	author = {Wierzba, A. and Reichl, U. and Turner, R. F. B. and Warren, R. A. J. and Kilburn, D. G.}
}
