@article {5225,
	title = {Reaction of human myoglobin and nitric oxide - Heme iron or protein sulfhydryl (S) nitrosation dependence on the absence or presence of oxygen},
	journal = {Journal of Biological Chemistry},
	volume = {276},
	number = {6},
	year = {2001},
	note = {ISI Document Delivery No.: 401HBTimes Cited: 33Cited Reference Count: 58},
	month = {Feb},
	pages = {3991-3998},
	type = {Article},
	abstract = {The amino acid sequence of human myoglobin (Mb) is similar to other mammalian Mb except for a unique cysteine residue at position 110 (Cys(110)). Anaerobic treatment of ferrous forms of wild-type human Rib, the C110A variant of human Mb or horse heart Mb, with either authentic NO or chemically derived NO in vitro yields heme-NO complexes as detected by electron paramagnetic resonance spectroscopy (EPR). By contrast, no EPR-detectable heme-NO complex was observed from the aerobic reactions of NO and either the ferric or oxy-Mb forms of wild-type human or horse heart myoglobins, Mass analyses of wild-type human Mb treated aerobically with NO indicated a mass increase of similar to 30 atomic mass units (i.e,, NO/Mb = 1 mol/mol), Mass analyses of the corresponding apoprotein after heme removal showed that NO was associated with the apoprotein fraction. New electronic maxima were detected at A(333) nm (epsilon = 3665 +/- 90 mol(-1) cm(-1); mean +/- S.D.) and A(545) nm (epsilon = 44 +/- 3 mol(-1) cm(-1)) in solutions of S-nitrosated wild-type human Mb (similar to S-nitrosoglutathione), Importantly, the sulfhydryl S-H stretch vibration for Cys(110) measured by Fourier transform infrared (nu similar to 2552 cm(-1)) was absent for both holo- and apo- forms of the wild-type human protein after aerobic treatment of the protein with NO. Together, these data indicate that the reaction of wild-type human Mb and NO yields either heme-NO or a novel S-nitrosated protein dependent on the oxidation state of the heme iron and the presence or absence of dioxygen.},
	keywords = {ELECTRON-SPIN-RESONANCE, GLUTATHIONE, HEMOGLOBIN, HUMAN SKELETAL-MUSCLE, METMYOGLOBIN, NITROSOTHIOLS, OXIDATION, RELAXING FACTOR, SMOOTH-MUSCLE, VASCULAR TONE},
	isbn = {0021-9258},
	url = {<Go to ISI>://000166921200038},
	author = {Witting, P. K. and Douglas, D. J. and Mauk, A. G.}
}
@article {3863,
	title = {Effects of low and high dose administration of bis(maltolato)oxovanadium(IV) on fa/fa Zucker rats},
	journal = {Canadian Journal of Physiology and Pharmacology},
	volume = {74},
	number = {9},
	year = {1996},
	note = {ISI Document Delivery No.: VX380Times Cited: 42Cited Reference Count: 57},
	month = {Sep},
	pages = {1001-1009},
	type = {Article},
	abstract = {The fatty Zucker rat, characterized by obesity, hyperinsulinemia, hyperlipidemia, and mild hyperglycemia, has been suggested as an animal model of non-insulin-dependent diabetes mellitus. The present study examined the chronic dose-dependent effect of bis(maltolato)oxovanadium(IV), a potent insulin mimetic, in this animal model of diabetes. Chronic (6 weeks) oral administration of bis(maltolato)oxovanadium(IV) (0.06 mmol . kg(-1). day(-1), low dose study) was effective in reducing the hyperinsulinemia associated with the fatty Zucker rat model (termination insulin: lean, 82.8 +/- 21.6; fatty, 732 +/- 89.4; fatty treated, 336 +/- 126.6 pmol/L; p < 0.05). Pancreatic perfusion data indicated a significant improvement in insulin secretory function in the fatty rats. The dose dependency of this relationship was observed in the high dose study (0.128 mmol . kg(-1). day(-1) for 14 weeks), wherein bis(maltolato)oxovanadium(IV) treatment restored plasma insulin levels in the fatty rats to lean levels (termination insulin: lean, 199.2 +/- 17.4; fatty, 660.6 +/- 12.6; fatty treated, 153.6 +/- 9.6 pmol/L; p < 0.05) and significantly improved insulin response to a glucose challenge. In addition, bis(maltolato)oxovanadium(IV) treatment (high dose study) ameliorated the age-dependent increase in blood pressure observed in fatty Zucker rats (systolic blood pressure: lean, 127 +/- 10; fatty, 176 +/- 5; fatty treated, 156 +/- 9 mmHg (1 mmHg = 133.3 Pa)). These data indicate that chronic oral administration of bis(maltolato)oxovanadium(IV) in the drinking water was effective in reducing hyperinsulinemia, insulin resistance, glucose intolerance, and hypertension in the fatty Zucker rat.},
	keywords = {bis(maltolato) oxovanadium(IV), BLOOD-PRESSURE, DEOXYCORTICOSTERONE-ACETATE, DIABETIC RATS, GLUCOSE-HOMEOSTASIS, HYPERTENSION, HYPERTENSIVE RATS, insulin resistance, LONG-TERM IMPROVEMENT, MUSCLE INSULIN RESISTANCE, OBESE RATS, ORAL VANADYL SULFATE, SMOOTH-MUSCLE, Zucker rat},
	isbn = {0008-4212},
	url = {<Go to ISI>://A1996VX38000001},
	author = {Yuen, V. G. and Pederson, R. A. and Dai, S. and Orvig, Chris and McNeill, J. H.}
}
