@article {4205,
	title = {Higher order equilibria and their effect on analyte migration behavior in capillary electrophoresis},
	journal = {Analytical Chemistry},
	volume = {70},
	number = {15},
	year = {1998},
	note = {ISI Document Delivery No.: 107VQTimes Cited: 38Cited Reference Count: 31},
	month = {Aug},
	pages = {3261-3270},
	type = {Article},
	abstract = {This paper presents a quantitative investigation into the effect of analyte-additive interactions on analyte migration behavior in capillary electrophoresis (CE) when both 1:1 and 1:2 stoichiometries are present. Equations based on the individual capacity factors for each interaction are derived to account for the effect of both first- and second-order equilibria. The analyte migration behavior is described using these equations with a full account of how the microscopic equilibrium constants and microscopic mobilities are combined to give the macroscopic values. The binding isotherms of interactions with both 1:1 and 1:2 stoichiometries are compared with those of a 1:1 stoichiometry. 4,4{\textquoteright}-Biphenol and 4-phenylphenol were chosen as analytes that undergo complexation with one and two hydroxypropyl-beta-cyclodextrin (HP-beta-CD) molecules; phenol was used as an analyte that interacts with only one HP-beta-CD molecule. The process of calculating higher order equilibrium constants and complex mobilities from the binding isotherms is demonstrated. The effects of experimental conditions, such as the additive concentration range and the number of data points, on the error in the calculated constants and the ability of the equations to accurately describe the experimental data are discussed. A comparison of the linear transformations of the binding isotherm with respect to their ability to detect higher order equilibria is made, and the advantage of using the capacity factor in CE is illustrated.},
	keywords = {AFFINITY, BETA-CYCLODEXTRIN, BINDING, CHIRAL SEPARATION, METAL-IONS, SELECTIVITY, SELECTORS, THEORETICAL ASPECTS, TIOCONAZOLE ENANTIOMERS, ZONE ELECTROPHORESIS},
	isbn = {0003-2700},
	url = {<Go to ISI>://000075232000034},
	author = {Bowser, M. T. and Chen, D. D. Y.}
}
@article {4209,
	title = {Properties of multivariate binding isotherms in capillary electrophoresis},
	journal = {Analytical Chemistry},
	volume = {70},
	number = {6},
	year = {1998},
	note = {ISI Document Delivery No.: ZC318Times Cited: 16Cited Reference Count: 30},
	month = {Mar},
	pages = {1076-1084},
	type = {Article},
	abstract = {When more than one complexation additive is used in capillary electrophoresis (CE), the migration behavior of an analyte can be described using contour plots and profile plots of the multivariate binding isotherms, At a certain concentration of one additive, the net electrophoretic mobility of the analyte is not affected by the concentration of the second additive, even though the second additive does alter the mobility of the analyte when used alone. The concentration of the first additive, in this situation, is defined as the dengsu concentration (dengsu means "same speed" in Chinese), The presence of a dengsu concentration for one additive is a strong indication that the second additive interacts with the analyte in a 1:1 (analyte-additive) stoichiometry, The binding isotherms in a profile plot can be used to unambiguously determine the binding stoichiometry of the analytes, as well as to determine the effect of interactions between the additives. The apparent complex mobilities obtained from the profile plots can also be used to determine whether there are interactions between the additives or whether the analyte can bind both additives at the same time.},
	keywords = {AFFINITY, BETA-CYCLODEXTRIN, CHIRAL SEPARATION, POLYETHERS, RESOLUTION, SELECTIVITY, THEORETICAL ASPECTS, TIOCONAZOLE ENANTIOMERS},
	isbn = {0003-2700},
	url = {<Go to ISI>://000072565200004},
	author = {Bowser, M. T. and Kranack, A. R. and Chen, D. D. Y.}
}
