@article {2137, title = {Synthesis and Enzymatic Incorporation of Modified Deoxyadenosine Triphosphates}, journal = {European Journal of Organic Chemistry}, number = {29}, year = {2008}, note = {ISI Document Delivery No.: 365YXTimes Cited: 7Cited Reference Count: 35Lam, Curtis Hipolito, Christopher Perrin, David M.}, month = {Oct}, pages = {4915-4923}, type = {Article}, abstract = {Several deoxyadenosine triphosphates containing modifications at the 8-position have been synthesized. Suitably protected 8-bromodeoxyadenosines were coupled with five imidazole-containing moieties by nucleophilic aromatic substitution or Sonagashira coupling to give modified nucleosides that were then triphosphorylated. Incorporation assays were performed for these modified residues with many commercially available DNA polymerases, and it was found that two of the modified dATPs could be effectively taken up as substrates by Sequenase V2.0. These two residues are candidates for substrates in combinatorial selections in the search for improved catalysis from DNAzymes. ((C) Wiley-VCH Verlag GmbH \& Co. KGaA, 69451 Weinheim, Germany, 2008)}, keywords = {AMINO-ACID, bioorganic chemistry, CATALYTIC REPERTOIRE, CLEAVING DNA ENZYME, DERIVATIVES, DNA, ENZYMES, FUNCTIONALITIES, HETEROCYCLES, IN-VITRO SELECTION, NUCLEIC-ACIDS, nucleotides, OLIGONUCLEOTIDES, PCR, POLYMERASES, SELEX}, isbn = {1434-193X}, url = {://000260447200009}, author = {Lam, C. and Hipolito, C. and Perrin,David M.} } @article {626, title = {Optimization of cellular nucleotide extraction and sample preparation for nucleotide pool analyses using capillary electrophoresis}, journal = {Journal of Chromatography B-Analytical Technologies in the Biomedical and Life Sciences}, volume = {788}, number = {1}, year = {2003}, note = {ISI Document Delivery No.: 664KCTimes Cited: 12Cited Reference Count: 28}, month = {May}, pages = {103-111}, type = {Article}, abstract = {Cell extraction and further sample preparation for nucleotide pool analysis using capillary electrophoresis was faster and simpler using volatile extraction solvents (e.g. organic solvents and de-ionized water) compared to the commonly applied acids dissolved in water (e.g. perchloric acid and trichloracetic acid). Temperature had to be controlled during the whole sample preparation process to prevent degradation, and extracts had to be cleaned from proteins and other large molecules prior to capillary electrophoretic analysis to improve reproducibility. Capillary electrophoresis using borate and cyclodextrins in the background electrolyte was used for determining 11 cellular nucleotides simultaneously. In order to optimize the assay, 0-100\% acetonitrile, 0-100\% ethanol, and 0-100\% methanol in de-ionized water were applied to extract nucleotides from mouse lymphoma cells, and nucleotide yields, recovery, and reproducibility were compared. The assay met the commonly accepted validation limits for biological fluids, if 20-80\% acetonitrile in water and 40-60\% ethanol in water were used as extraction solvents. (C) 2003 Elsevier Science B.V. All rights reserved.}, keywords = {CELLS, dynamic pH junction, FLUIDS, MIGRATION BEHAVIOR, NUCLEOSIDES, nucleotides, PERFORMANCE LIQUID-CHROMATOGRAPHY, QUANTITATIVE-ANALYSIS, SEPARATION, TISSUES, TRIPHOSPHATES}, isbn = {1570-0232}, url = {://000182059400012}, author = {Grob, M. K. and O{\textquoteright}Brien, K. and Chu, J. J. and Chen, D. D. Y.} } @article {4499, title = {UDP-glucose analogues as inhibitors and mechanistic probes of UDP-glucose dehydrogenase}, journal = {Journal of Organic Chemistry}, volume = {64}, number = {26}, year = {1999}, note = {ISI Document Delivery No.: 271NJTimes Cited: 15Cited Reference Count: 25}, month = {Dec}, pages = {9487-9492}, type = {Article}, abstract = {UDP-glucose dehydrogenase catalyzes the NAD(+)-dependent 2-fold oxidation of UDP-glucose to give UDP-glucuronic acid. The putative aldehyde intermediate is not released from the active site and is presumably tightly bound. We have prepared UDP-7-deoxy-alpha-D-gluco-hept-6-ulopyranose 5, that contains a methyl ketone at C-6 and cannot be further oxidized by the enzyme. Ketone 5 was found to be a competitive inhibitor of the dehydrogenase from Streptococcus pyogenes with a K-I value of 6.7 mu M. We have also prepared the secondary alcohols UDP-6S-6C-methylglucose, 4a, and UDP-6R-6C-methylglucose, 4b. Compound 4a, but not 4b, was found to be a slow substrate for the dehydrogenase and was converted into the ketone inhibitor 5. This is consistent with the notion that the pro-R hydride is transferred in the first oxidation step of the normal enzymatic reaction.}, keywords = {EFFICIENT SYNTHESIS, FUCOSE, GROUP-A STREPTOCOCCI, nucleotides, POLYSACCHARIDE, VIRULENCE}, isbn = {0022-3263}, url = {://000084600100019}, author = {Campbell, R. E. and Tanner, M. E.} } @article {3923, title = {Uridine diphospho-alpha-D-gluco-hexodialdose: Synthesis and kinetic competence in the reaction catalyzed by UDP-glucose dehydrogenase}, journal = {Angewandte Chemie-International Edition in English}, volume = {36}, number = {13-14}, year = {1997}, note = {ISI Document Delivery No.: XR042Times Cited: 9Cited Reference Count: 29}, month = {Aug}, pages = {1520-1522}, type = {Article}, keywords = {carbohydrates, enzyme catalysis, GROUP-A STREPTOCOCCI, intermediates, nucleotides, VIRULENCE}, isbn = {0570-0833}, url = {://A1997XR04200042}, author = {Campbell, R. E. and Tanner, M. E.} } @article {4101, title = {Variance contributed by pressure induced injection in capillary electrophoresis}, journal = {Journal of Chromatography A}, volume = {767}, number = {1-2}, year = {1997}, note = {ISI Document Delivery No.: WY814Times Cited: 14Cited Reference Count: 12}, month = {Apr}, pages = {205-216}, type = {Article}, abstract = {The contribution of variance from a pressure injection to the final analyte peak in capillary electrophoresis (CE) is studied quantitatively. The band broadening process of CE can be described by the product of the Laplace transformed contributing factors; the inverse transform of the product gives the final peak shape which is the convolution of the original functions. The CE process is, therefore, similar to the chromatographic processes described by Sternberg. Based on the additivity of the variance, the injection induced variance is transferred directly to the final peak. The differences between the concepts of band broadening and peak broadening are discussed. Because the injection length from a pressure induced injection is the same for all analytes, and the migration rates during the CE separation are different for these analytes, the variance contributed by injection is larger for slower migrating analytes. The pressure forced flow generates a parabolic component in an injected sample plug, and this should also be considered in calculating the total injection length (tau). The variance is (1/16)tau(2) for a sample plug with a Gaussian concentration profile, and (1/12)tau(2) for one with a rectangular profile. It is demonstrated that the total variance of an analyte peak increases at the same rate as the injection variance. The difference between the total variance and the injection variance is contributed by longitudinal diffusion and other factors.}, keywords = {band broadening, injection methods, NUCLEOSIDES, nucleotides, peak broadening, peak shape, ZONE ELECTROPHORESIS}, isbn = {0021-9673}, url = {://A1997WY81400023}, author = {Peng, X. J. and Chen, D. D. Y.} }