@article {335,
	title = {The power distribution advantage of fiber-optic coupled ultraviolet resonance Raman spectroscopy for bioanalytical and biomedical applications},
	journal = {Journal of Raman Spectroscopy},
	volume = {33},
	number = {7},
	year = {2002},
	note = {ISI Document Delivery No.: 580KBTimes Cited: 18Cited Reference Count: 51},
	month = {Jul},
	pages = {503-510},
	type = {Article},
	abstract = {Fiber-optic coupled ultraviolet resonance Raman spectroscopy (FO-UVRRS) of photosensitive biological samples is discussed in the context of both clinical and basic medical research applications. The fiberoptic probes are designed specifically for resonance Raman spectroscopy and offer a power distribution advantage over conventional focusing UVRR spectrometers that allows the use of higher power levels without increasing the risk of photo-damage. A typical fiber-optic probe using a 600 pm core diameter fiber for illumination of the sample allows an increase in power at the sample of over an order of magnitude while maintaining the same power density, and therefore the same level of safety, as a typical conventional UVRR spectrometer. This allows high-quality spectra to be easily obtained in 10 s. Spectra of representative biological samples using high power levels without damage are presented, including the study of a photosensitive enzyme-substrate system under anaerobic conditions. Copyright (C) 2002 John Wiley Sons, Ltd.},
	keywords = {ACTIVATION, AROMATIC-AMINO-ACIDS, BACTERIA, DEEP UV, ENZYMES, LASER EXCITATION, PROBE, SIMPLICITY, SPECTRA, TISSUE},
	isbn = {0377-0486},
	url = {<Go to ISI>://000177232800003},
	author = {Barbosa, C. J. and Vaillancourt, F. H. and Eltis, L. D. and Blades, M. W. and Turner, R. F. B.}
}
@article {335,
	title = {The power distribution advantage of fiber-optic coupled ultraviolet resonance Raman spectroscopy for bioanalytical and biomedical applications},
	journal = {Journal of Raman Spectroscopy},
	volume = {33},
	number = {7},
	year = {2002},
	note = {ISI Document Delivery No.: 580KBTimes Cited: 18Cited Reference Count: 51},
	month = {Jul},
	pages = {503-510},
	type = {Article},
	abstract = {Fiber-optic coupled ultraviolet resonance Raman spectroscopy (FO-UVRRS) of photosensitive biological samples is discussed in the context of both clinical and basic medical research applications. The fiberoptic probes are designed specifically for resonance Raman spectroscopy and offer a power distribution advantage over conventional focusing UVRR spectrometers that allows the use of higher power levels without increasing the risk of photo-damage. A typical fiber-optic probe using a 600 pm core diameter fiber for illumination of the sample allows an increase in power at the sample of over an order of magnitude while maintaining the same power density, and therefore the same level of safety, as a typical conventional UVRR spectrometer. This allows high-quality spectra to be easily obtained in 10 s. Spectra of representative biological samples using high power levels without damage are presented, including the study of a photosensitive enzyme-substrate system under anaerobic conditions. Copyright (C) 2002 John Wiley Sons, Ltd.},
	keywords = {ACTIVATION, AROMATIC-AMINO-ACIDS, BACTERIA, DEEP UV, ENZYMES, LASER EXCITATION, PROBE, SIMPLICITY, SPECTRA, TISSUE},
	isbn = {0377-0486},
	url = {<Go to ISI>://000177232800003},
	author = {Barbosa, C. J. and Vaillancourt, F. H. and Eltis, L. D. and Blades, M. W. and Turner, R. F. B.}
}
@article {7358,
	title = {MONOMER AND EXCIMER FLUORESCENCE OF HORSE PLASMA GELSOLIN LABELED WITH N-(1-PYRENYL)IODOACETAMIDE},
	journal = {Biochemistry and Cell Biology-Biochimie Et Biologie Cellulaire},
	volume = {70},
	number = {7},
	year = {1992},
	note = {ISI Document Delivery No.: JP667Times Cited: 3Cited Reference Count: 27},
	month = {Jul},
	pages = {573-578},
	type = {Article},
	abstract = {Horse plasma gelsolin was labelled with the sulfhydryl-specific fluorescent reagent N-(1-pyrenyl)iodoacetamide. The level of incorporation of probe was 1.6 +/- 0.3 mol pyrene/mol gelsolin. The circular dichroism spectrum of pyrenyl-gelsolin and its ability to interact with muscle actin were not different from that found for unmodified gelsolin. The emission from pyrenyl-gelsolin was dominated by a broad emission band centred near 483 nm, characteristic of the presence of pyrene excimers. Analysis of excitation spectra for the monomer and excimer-type fluorescence suggested that ground-state interactions may occur between adjacent pyrenes in the gelsolin structure. In the case either of excimer formation or of ground-state pyrene-pyrene interactions in doubly labelled gelsolin molecules, the modified Cys residues must be in close proximity in the folded protein structure. Thermal denaturation of gelsolin could be monitored by observing the decrease in excimer emission that accompanied heating and unfolding of the tertiary structure. While heat treatment alone did not eliminate excimer fluorescence, digestion of gelsolin with chymotrypsin completely abolished such emission. Also, pyrenyl-gelsolin prepared and studied in 6 M guanidine-HCl exhibited fluorescence characteristic of pyrene monomers exclusively.},
	keywords = {ACTIN-FILAMENTS, BINDING, CIRCULAR-DICHROISM, denaturation, FLUORESCENCE LIFETIME, gelsolin, POLYMERIZATION, PROBE, PROTEIN, PYRENE EXCIMER FLUORESCENCE, PYRENE-ACTIN, STATE, thermal, TROPOMYOSIN},
	isbn = {0829-8211},
	url = {<Go to ISI>://A1992JP66700008},
	author = {Silva, B. E. R. and Koepf, E. K. and Burtnick, L. D. and Turro, N. J.}
}
