@article {ELPS:ELPS6157,
	title = {Improved sensitivity by post-column chemical environment modification of CE-ESI-MS using a flow-through microvial interface},
	journal = {ELECTROPHORESIS},
	volume = {38},
	year = {2017},
	month = {06/2017},
	pages = {1644{\textendash}1648},
	type = {Research},
	keywords = {capillary electrophoresis, electrospray ionization, MASS SPECTROMETRY, Peak symmetry, Post-column modification, SENSITIVITY},
	issn = {1522-2683},
	doi = {10.1002/elps.201600545},
	url = {http://dx.doi.org/10.1002/elps.201600545},
	author = {Risley, Jessica May and Chen, David Da Yong}
}
@article {2650,
	title = {Propofol protects against hydrogen peroxide-induced injury in cardiac H9c2 cells via Akt activation and Bcl-2 up-regulation},
	journal = {Biochemical and Biophysical Research Communications},
	volume = {389},
	number = {1},
	year = {2009},
	note = {ISI Document Delivery No.: 555SMTimes Cited: 4Cited Reference Count: 26Wang, Baohua Shravah, Jayant Luo, Honglin Raedschelders, Koen Chen, David D. Y. Ansley, David M.},
	month = {Nov},
	pages = {105-111},
	type = {Article},
	abstract = {<p>Propofol is a widely used intravenous anesthetic agent with antioxidant properties secondary to its phenol based chemical structure. Treatment with propofol has been found to attenuate oxidative stress and prevent ischemia/reperfusion injury in rat heart. Here, we report that propofol protects cardiac H9c2 cells from hydrogen peroxide (H2O2)-induced injury by triggering the activation of Akt and a parallel up-regulation of Bcl-2. We show that pretreatment with propofol significantly protects against H2O2-induced injury. We further demonstrate that propofol activates the PI3K-Akt signaling pathway. The protective effect of propofol on H2O2-induced injury is reversed by PI3K inhibitor wortmannin, which effectively suppresses propofol-induced activation of Akt, up-regulation of Bcl-2, and protection from apoptosis. Collectively, our results reveal a new mechanism by which propofol inhibits H2O2-induced injury in cardiac H9c2 cells, supporting a potential application of propofol as a preemptive cardioprotectant in clinical settings such as coronary bypass surgery. (C) 2009 Elsevier Inc. All rights reserved.</p>},
	keywords = {15-F-2T-ISOPROSTANE FORMATION, Akt, antioxidant capacity, apoptosis, Bcl-2, CARDIOMYOCYTES, ENDOTHELIAL-CELLS, H9c2 cells, INJURY, KINASE-C, OXIDATIVE STRESS, Propofol, RAT-HEART, REDUCES APOPTOSIS, REPERFUSION, SENSITIVITY, SURVIVAL},
	isbn = {0006-291X},
	doi = {doi: 10.1016/j.bbrc.2009.08.097},
	url = {<Go to ISI>://000274534900020},
	author = {Wang, B. H. and Shravah, J. and Luo, H. L. and Raedschelders, K. and Chen, D. D. Y. and Ansley, D. M.}
}
@article {1237,
	title = {Effects of solvent flow, dopant flow, and lamp current on dopant-assisted atmospheric pressure photoionization (DA-APPI) for LC-MS. Ionization via proton transfer},
	journal = {Journal of the American Society for Mass Spectrometry},
	volume = {16},
	number = {8},
	year = {2005},
	note = {ISI Document Delivery No.: 952BFTimes Cited: 42Cited Reference Count: 37},
	month = {Aug},
	pages = {1275-1290},
	type = {Article},
	abstract = {In this paper, the effects of solvent flow, dopant flow, and lamp power on proton transfer ionization in dopant-assisted (DA) atmospheric pressure photoionization (APPI) are investigated. A broad theoretical framework is presented, describing the primary photoionization process, the formation of protonated-solvent cluster ions, and the balance between analyte ion creation via proton transfer and loss via recombination. The principal experimental test system utilized methanol as the solvent, toluene as the dopant, and acridine as the analyte. Comparisons are made between acridine and a less basic compound, 9-methylanthracene (9-MA). Experimental determinations of the trends in the analyte MH+ signal and the total ion current (TIC) with variations in the subject parameters are provided. Experimental results and theory demonstrate that both the analyte signal and the TIC approach asymptotic limits with increases in dopant flow and/or lamp current (two factors which dictate the rate of photoion generation). The data show that these limits are lowered at higher solvent flow rates. These results are attributed to the recombination loss process, the rate of which increases with the second power of ion concentration. We deduce that the recombination rate constant increases with solvent flow rate, a consequence of the growth of ion-solvent clusters. Cluster growth is also believed to be a factor in the dramatic loss of sensitivity for 9-MA that occurs as the solvent flow is raised, because larger protonated-solvent cluster ions have greater solvation energies and may be unreactive with compounds having low gas-phase basicity and/or low solvation energy.},
	keywords = {DETECTOR, GAS-PHASE, ION-SOURCE, LIQUID-CHROMATOGRAPHY, MASS-SPECTROMETRY, METHANOL, PLASMA, SENSITIVITY, SOLVATION, SYSTEM},
	isbn = {1044-0305},
	url = {<Go to ISI>://000230975700009},
	author = {Robb, D. B. and Blades, M. W.}
}
@article {4924,
	title = {Collision-induced dissociation of ions within the orifice-skimmer region of an electrospray mass spectrometer},
	journal = {Analytical Chemistry},
	volume = {72},
	number = {4},
	year = {2000},
	note = {ISI Document Delivery No.: 285HETimes Cited: 38Cited Reference Count: 33},
	month = {Feb},
	pages = {791-799},
	type = {Article},
	abstract = {An equation was derived to describe the variation of the gas number density within the region between the orifice and the skimmer of an electrospray ionization mass spectrometer. The equation was used to develop a semiquantitative model to predict the value of orifice voltages that lead to ion fragmentation within this region. This model made it possible to predict the types of solvent adducts observed for analytes at various orifice voltages. In addition, it is shown that a small number of high-energy collisions is equally effective for collision-induced dissociation as compared to a large number of low-energy collisions. Finally, this model is tested with different background electrolyte solutions and a different electrospray mass spectrometer. It is demonstrated that controlled fragmentation studies can be performed on single-quadrupole mass spectrometers, and the proposed model gives a reasonable description of the fragmentation process in both spectrometers.},
	keywords = {capillary electrophoresis, CYTOCHROME-C, DYNAMICS, IDENTIFICATION, INTERFACE, IONIZATION, LIQUID-CHROMATOGRAPHY, PROTEINS, SENSITIVITY, SEPARATION},
	isbn = {0003-2700},
	url = {<Go to ISI>://000085383000019},
	author = {Schneider, B. B. and Chen, D. D. Y.}
}
@article {4578,
	title = {The cellulose-binding domains from Cellulomonas fimi beta-1,4-glucanase CenC bind nitroxide spin-labeled cellooligosaccharides in multiple orientations},
	journal = {Journal of Molecular Biology},
	volume = {287},
	number = {3},
	year = {1999},
	note = {ISI Document Delivery No.: 184RGTimes Cited: 38Cited Reference Count: 46},
	month = {Apr},
	pages = {609-625},
	type = {Article},
	abstract = {The N-terminal cellulose-binding domains CBDN1 and CBDN2 from Cellulomonas fimi cellulase CenC each adopt a jelly-roll beta-sandwich structure with a cleft into which amorphous cellulose and soluble cellooligosaccharides bind. To determine the orientation of the sugar chain within these binding clefts, the association of TEMPO (2,2,6,6-tetramethylpiperidine-1-oxyl-4-yl) spin-labeled derivatives of cellotriose and cellotetraose with isolated CBDN1 and CBDN2 was studied using heteronuclear H-1-N-15 NMR spectroscopy. Quantitative binding measurements indicate that the TEMPO moiety does not significantly perturb the affinity of the cellooligo-saccharide derivatives for the CBDs. The paramagnetic enhancements of the amide H-1(N) longitudinal (Delta R-1) and transverse (Delta R-2) relaxation rates were measured by comparing the effects of TEMPO-cellotetraose in its nitroxide (oxidized) and hydroxylamine (reduced) forms on the two CBDs. The bound spin-label affects most significantly the relaxation rates of amides located at both ends of the sugar-binding cleft of each CBD. Similar results are observed with TEMPO-cellotriose bound to CBDN1. This demonstrates that the TEMPO-labeled cellooligosaccharides, and by inference strands of amorphous cellulose, can associate with CBDN1 and CBDN2 in either orientation across their beta-sheet binding clefts. The ratio of the association constants for binding in each of these two orientations is estimated to be within a factor of five to tenfold. This finding is consistent with the approximate symmetry of the hydrogen-bonding groups on both the cellooligosaccharides and the residues forming the binding clefts of the CenC CBDs. (C) 1999 Academic Press.},
	keywords = {CELLULASES, ENDOGLUCANASE, GLYCOSYNTHASE, MAGNETIC-RESONANCE SPECTROSCOPY, NMR, protein-carbohydrate interaction, PROTEINS, SENSITIVITY, SITE, SPECIFICITY, spin label},
	isbn = {0022-2836},
	url = {<Go to ISI>://000079626300013},
	author = {Johnson, P. E. and Brun, E. and Mackenzie, L. F. and Withers, S. G. and McIntosh, L. P.}
}
@article {3357,
	title = {CHARACTERIZATION OF AN OLIGONUCLEOTIDE-BINDING FLUORESCENT LIGAND FOR APPLICATION IN AFFINITY PURIFICATION OF DSDNA},
	journal = {Biotechnology and Bioengineering},
	volume = {48},
	number = {1},
	year = {1995},
	note = {ISI Document Delivery No.: RU627Times Cited: 1Cited Reference Count: 39},
	month = {Oct},
	pages = {25-35},
	type = {Article},
	abstract = {The fluorescent probe PO-PRO-3 was investigated as a potential ligand for the affinity immobilization and purification of genomic or plasmid DNA fragments. Affinities and mechanisms for PO-PRO-3 binding to superhelical and linearized pUC 18 plasmid DNA were examined through measurement of binding isotherms, continuous-variation analysis, and DNA titrations. In addition, the effects of DNA conformation, protein and RNA contaminants, solvent polarity, and ionic strength are examined with the aim of optimizing binding and elution conditions and of assigning limits to the range of applicability of the affinity purification. (C) 1995 John Wiley \& Sons, Inc.},
	keywords = {ANTHRACYCLINE, antibiotics, BIO-POLYMERS, DNA AMPLIFICATION, DNA PURIFICATION, DNA-BINDING FLUOROPHORE, DYES, FLUORESCENT LIGAND, POLYMERASE CHAIN-REACTION, SENSITIVITY},
	isbn = {0006-3592},
	url = {<Go to ISI>://A1995RU62700004},
	author = {Haynes, C. A. and Sherwood, C. S. and Turner, R. F. B.}
}
@article {2940,
	title = {GLUCOSE-LOWERING EFFECTS OF A NEW ORGANIC VANADIUM COMPLEX, BIS(MALTOLATO)OXOVANADIUM(IV)},
	journal = {Canadian Journal of Physiology and Pharmacology},
	volume = {71},
	number = {3-4},
	year = {1993},
	note = {ISI Document Delivery No.: LN775Times Cited: 84Cited Reference Count: 39},
	month = {Mar-Apr},
	pages = {263-269},
	type = {Article},
	abstract = {Inorganic vanadium has been shown, both in vivo and in vitro, to have insulin-mimetic properties. A new organic vanadium complex, bis(maltolato)oxovanadium(IV) (BMOV), was developed to increase the absorption of vanadium from the gastrointestinal tract, thereby reducing the dose of vanadium necessary to produce glucose-lowering effects. BMOV was administered in the drinking water for 25 weeks to control and streptozotocin-induced diabetic, male Wistar rats. BMOV treatment produced a stable euglycemic state in 70\% of diabetic treated animals. The other 30\% of the diabetic treated animals demonstrated fluctuations in glucose control over the entire study period. The initial effective dose of BMOV was 0.45 mmol/kg, which decreased to an effective maintenance dose of 0. 18 mmol/kg, significantly lower than the dose of inorganic vanadium salts used in previous studies. BMOV treatment did significantly reduce fluid consumption levels in control treated animals after 10 weeks of therapy; however, the food consumption for control treated animals was only intermittently lower than that for controls. Plasma cholesterol and triglyceride levels were normalized with BMOV treatment for all diabetic treated animals, without a concomitant increase in plasma insulin levels. An oral glucose tolerance test demonstrated that glucose homeostasis in control-treated animals occurred at significantly lower plasma insulin levels than in control animals. BMOV effectively produced the glucose-lowering effects at significantly lower dose than previously used for inorganic vanadium salts, without any overt signs of toxicity.},
	keywords = {ACTIVATION, ADIPOCYTES, BIS(MALTOLATO)OXOVANADIUM(IV), BLOOD-GLUCOSE, DIABETES TREATMENT, DIABETIC RATS, GLYCOLYSIS, INSULIN, SENSITIVITY, SKELETAL-MUSCLE, STIMULATION, streptozotocin-induced diabetes, VANADATE},
	isbn = {0008-4212},
	url = {<Go to ISI>://A1993LN77500013},
	author = {Yuen, V. G. and Orvig, Chris and McNeill, J. H.}
}
