@article {4703,
	title = {Electrostatically mediated interactions between cationic lipid-DNA particles and an anionic surface},
	journal = {Archives of Biochemistry and Biophysics},
	volume = {366},
	number = {1},
	year = {1999},
	note = {ISI Document Delivery No.: 202JKTimes Cited: 7Cited Reference Count: 29},
	month = {Jun},
	pages = {31-39},
	type = {Article},
	abstract = {In an effort to model the interaction of lipid-based DNA delivery systems with anionic surfaces, such as a cell membrane, we have utilized microelectrophoresis to characterize how electrokinetic measurements can provide information on surface charge and binding characteristics. We have established that cationic lipids, specifically N-N-dioleoyl-N,N-dimethylammonium chloride (DODAC), incorporated into liposomes prepared with I,2-dioleoyl-i-glycero-3-phosphoethanolamine (DOPE) or 1,2-dioleoyl-sn-glycero-3-phosphocholine (DOPC) at 50 mol\%, change the inherent electrophoretic mobility of anionic latex polystyrene beads. Self-assembling lipid-DNA particles (LDPs), prepared at various cationic lipid to negative DNA phosphate charge ratios, effected no changes in bead mobility when the LDP charge ratio (+/-) was equal to or less than 1. Increasing the LDP concentration in a solution of 0.1\% (w/v) anionic beads resulted in a charge reversal effect when a net charge of LDP to total bead charge ratio (+/-) of 1:1 was observed. LDP formulations, utilizing either DOPE or DOPC, showed similar titration profiles with a charge reversal observed at a 1:1 net LDP to bead charge ratio (+/-). It was confirmed through centrifugation studies that the DNA in the LDP was associated with the anionic latex beads through electrostatic interactions. LDP binding, rather than the binding of dissociated cationic lipids, resulted in the observed electrophoretic mobility changes of the anionic latex beads. (C) 1999 Academic Press.},
	keywords = {BILAYERS, CELLS, ELECTROSTATIC INTERACTIONS, FUSION, GENE-TRANSFER, lipid-DNA particles, lipoplex, LIPOSOMES, microelectrophoresis, PHOSPHATIDYLETHANOLAMINE, PLASMID DNA, VESICLES},
	isbn = {0003-9861},
	url = {<Go to ISI>://000080648400005},
	author = {Wong, F. M. P. and Bally, M. B. and Brooks, D. E.}
}
@article {3763,
	title = {Driving forces for DNA adsorption to silica in perchlorate solutions},
	journal = {Journal of Colloid and Interface Science},
	volume = {181},
	number = {2},
	year = {1996},
	note = {ISI Document Delivery No.: UZ536Times Cited: 112Cited Reference Count: 43},
	month = {Aug},
	pages = {635-644},
	type = {Article},
	abstract = {The adsorption of both plasmid and chromosomal duplex DNA to silica is investigated with the aim of determining the dominant forces involved in the binding reaction. Changes in the initial slopes and plateau values of adsorption isotherms for DNA on microcrystalline silica particles are used to establish the sensitivity of the binding reaction to ionic strength, temperature and pH, and DNA size and conformation. Binding is driven by an increase in entropy, with little or no enthalpic contribution. Adsorption isotherm results indicate that three effects, namely: (i) shielded intermolecular electrostatic forces, (ii) dehydration of the DNA and silica surfaces, and (iii) intermolecular hydrogen bond formation in the DNA-silica contact layer, make the dominant contributions to the overall driving force for adsorption. (C) 1996 Academic Press, Inc.},
	keywords = {adsorption isotherms, ARRAYS, CHIPS, CONFORMATION, DNA, DNA adsorption, DNA binding, DNA PURIFICATION, DNA-silica interactions, ELECTROPHORESIS, GLOBULAR-PROTEINS, PLASMID DNA, PURIFICATION, SURFACES, TRANSITION},
	isbn = {0021-9797},
	url = {<Go to ISI>://A1996UZ53600031},
	author = {Melzak, K. A. and Sherwood, C. S. and Turner, R. F. B. and Haynes, C. A.}
}
